Journal article
D-methionine pre-loading reduces both noise-induced permanent threshold shift and outer hair cell loss in the chinchilla
International journal of audiology, Vol.52(12), pp.801-807
12/01/2013
DOI: 10.3109/14992027.2013.840933
PMID: 24175619
Abstract
Objective: This study tested multiple dosing epochs of pre-loaded D-methionine (D-met) for otoprotection from noise-induced hearing loss (NIHL). Design: Auditory brainstem response (ABR) thresholds were measured at baseline, 1 day, and 21 days following a 6-hour 105 dB sound pressure level (SPL) octave band noise (OBN) exposure. Outer hair cell (OHC) counts were measured after day 21 sacrifice. Study sample: Three groups of five Chinchillas laniger each were given a 2-day regimen comprising five doses of D-met (200 mg/kg/dose) intraperitoneally (IP) starting 2, 2.5, or 3 days prior to noise exposure. A control group (n = 5) received five doses of equivalent volume saline IP starting 2.5 days prior to noise exposure. Results: ABR threshold shifts from baseline to day-21 post-noise exposure were reduced in all D-met groups versus controls, reaching significance (p < 0.05) in the 3-day group. D-met groups showed reduced OHC loss relative to controls at day-21 post-noise exposure, reaching significance (p < 0.05) at all frequency regions in the 3-day group and at the 2, 4, and 8 kHz frequency regions in the 2.5-day group. Conclusions: D-met administration in advance of noise-exposure, without further administration, significantly protects from noise-induced ABR threshold shift and OHC loss.
Details
- Title: Subtitle
- D-methionine pre-loading reduces both noise-induced permanent threshold shift and outer hair cell loss in the chinchilla
- Creators
- Alexander D. Claussen - Southern Illinois University School of MedicineDaniel J. Fox - Southern Illinois University School of MedicineXin C. Yu - Southern Illinois University School of MedicineRobert P. Meech - Southern Illinois University School of MedicineSteven J. Verhulst - Southern Illinois University School of MedicineTim L. Hargrove - Southern Illinois University School of MedicineKathleen C. M. Campbell - Southern Illinois University School of Medicine
- Resource Type
- Journal article
- Publication Details
- International journal of audiology, Vol.52(12), pp.801-807
- Publisher
- Taylor & Francis
- DOI
- 10.3109/14992027.2013.840933
- PMID
- 24175619
- ISSN
- 1499-2027
- eISSN
- 1708-8186
- Language
- English
- Date published
- 12/01/2013
- Academic Unit
- Otolaryngology
- Record Identifier
- 9984366363602771
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