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D1 dopamine receptor dDA1 is required in the mushroom body neurons for aversive and appetitive learning in Drosophila
Journal article   Open access   Peer reviewed

D1 dopamine receptor dDA1 is required in the mushroom body neurons for aversive and appetitive learning in Drosophila

Young-Cho Kim, Hyun-Gwan Lee and Kyung-An Han
The Journal of neuroscience, Vol.27(29), pp.7640-7647
07/18/2007
DOI: 10.1523/JNEUROSCI.1167-07.2007
PMID: 17634358
url
https://doi.org/10.1523/JNEUROSCI.1167-07.2007View
Published (Version of record) Open Access

Abstract

Drosophila has robust behavioral plasticity to avoid or prefer the odor that predicts punishment or food reward, respectively. Both types of plasticity are mediated by the mushroom body (MB) neurons in the brain, in which various signaling molecules play crucial roles. However, important yet unresolved molecules are the receptors that initiate aversive or appetitive learning cascades in the MB. We have shown previously that D1 dopamine receptor dDA1 is highly enriched in the MB neuropil. Here, we demonstrate that dDA1 is a key receptor that mediates both aversive and appetitive learning in pavlovian olfactory conditioning. We identified two mutants, dumb1 and dumb2, with abnormal dDA1 expression. When trained with the same conditioned stimuli, both dumb alleles showed negligible learning in electric shock-mediated conditioning while they exhibited moderately impaired learning in sugar-mediated conditioning. These phenotypes were not attributable to anomalous sensory modalities of dumb mutants because their olfactory acuity, shock reactivity, and sugar preference were comparable to those of control lines. Remarkably, the dumb mutant's impaired performance in both paradigms was fully rescued by reinstating dDA1 expression in the same subset of MB neurons, indicating the critical roles of the MB dDA1 in aversive as well as appetitive learning. Previous studies using dopamine receptor antagonists implicate the involvement of D1/D5 receptors in various pavlovian conditioning tasks in mammals; however, these have not been supported by the studies of D1- or D5-deficient animals. The findings described here unambiguously clarify the critical roles of D1 dopamine receptor in aversive and appetitive pavlovian conditioning.
 Animals, Genetically Modified Appetitive Behavior - physiology Drosophila Male Avoidance Learning - physiology Mutation - genetics Behavior, Animal Animals Analysis of Variance Receptors, Dopamine D1 - physiology Neurons - physiology Conditioning, Classical Mushroom Bodies - cytology Female Learning Disorders - genetics Drosophila Proteins - genetics Receptors, Dopamine D1 - genetics

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