Journal article
DNA-Functionalized Ti3C2Tx MXenes for Selective and Rapid Detection of SARS-CoV-2 Nucleocapsid Gene
ACS applied nano materials, Vol.5(5), pp.1902-1910
12/30/2021
DOI: 10.1021/acsanm.1c03520
Abstract
Coronavirus disease 2019 (COVID-19) is an emerging human infectious disease caused by severe acute respiratory syndrome 2 (SARS-CoV-2, initially called novel coronavirus 2019-nCoV) virus. Thus, an accurate and specific diagnosis of COVID-19 is urgently needed for effective point-of-care detection and disease management. The reported promise of two-dimensional (2D) transition-metal carbides (Ti3C2Tx MXene) for biosensing owing to a very high surface area, high electrical conductivity, and hydrophilicity informed their selection for inclusion in functional electrodes for SARS-CoV-2 detection. Here, we demonstrate a new and facile functionalization strategy for Ti3C2Tx with probe DNA molecules through noncovalent adsorption, which eliminates expensive labeling steps and achieves sequence-specific recognition. The 2D Ti3C2Tx functionalized with complementary DNA probes shows a sensitive and selective detection of nucleocapsid (N) gene from SARS-CoV-2 through nucleic acid hybridization and chemoresistive transduction. The fabricated sensors are able to detect the SARS-CoV-2 N gene with sensitive and rapid response, a detection limit below 10(5) copies/mL in saliva, and high specificity when tested against SARS-CoV-1 and MERS. We hypothesize that the MXenes' interlayer spacing can serve as molecular sieving channels for hosting organic molecules and ions, which is a key advantage to their use in biomolecular sensing.
Details
- Title: Subtitle
- DNA-Functionalized Ti3C2Tx MXenes for Selective and Rapid Detection of SARS-CoV-2 Nucleocapsid Gene
- Creators
- Winston Yenyu Chen - Purdue University West LafayetteHang Lin - Purdue University West LafayetteAmit Kumar Barui - Purdue University West LafayetteAna Maria Ulloa Gomez - Purdue University West LafayetteMichael K. Wendt - Purdue University West LafayetteLia A. Stanciu - Purdue University West Lafayette
- Resource Type
- Journal article
- Publication Details
- ACS applied nano materials, Vol.5(5), pp.1902-1910
- Publisher
- Amer Chemical Soc
- DOI
- 10.1021/acsanm.1c03520
- ISSN
- 2574-0970
- eISSN
- 2574-0970
- Number of pages
- 9
- Grant note
- 2027554 / NSF/CBET; National Science Foundation (NSF)
- Language
- English
- Date published
- 12/30/2021
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984460329102771
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