Journal article
DNA Packaging Specificity in the λ-Like Phages: Gifsy-1
Molecular microbiology, Vol.122(4), pp.491-503
10/2024
DOI: 10.1111/mmi.15306
PMID: 39233649
Appears in UI Libraries Support Open Access
Abstract
DNA viruses recognize viral DNA and package it into virions. Specific recognition is needed to distinguish viral DNA from host cell DNA. The lambda-like Escherichia coli phages are interesting and good models to examine genome packaging by large DNA viruses. Gifsy-1 is a lambda-like Salmonella phage. Gifsy-1's DNA packaging specificity was compared with those of closely related phages lambda, 21, and N15. In vivo packaging studies showed that a Gifsy-1-specific phage packaged lambda DNA at ca. 50% efficiency and lambda packages Gifsy-1-specific DNA at &SIM30% efficiency. The results indicate that Gifsy-1 and lambda share the same DNA packaging specificity. N15 is also shown to package Gifsy-1 DNA. Phage 21 fails to package lambda, N15, and Gifsy-1-specific DNAs; the efficiencies are 0.01%, 0.01%, and 1%, respectively. A known incompatibility between the 21 helix-turn-helix motif and cosB lambda is proposed to account for the inability of 21 to package Gifsy-1 DNA. A model is proposed to explain the 100-fold difference in packaging efficiency between lambda and Gifsy-1-specific DNAs by phage 21. Database sequences of enteric prophages indicate that phages with Gifsy-1's DNA packaging determinants are confined to Salmonella species. Similarly, prophages with lambda DNA packaging specificity are rarely found in Salmonella. It is proposed that lambda and Gifsy-1 have diverged from a common ancestor phage, and that the differences may reflect adaptation of their packaging systems to host cell differences.
Details
- Title: Subtitle
- DNA Packaging Specificity in the λ-Like Phages: Gifsy-1
- Creators
- Michael Feiss - University of Iowa, Microbiology and ImmunologyJean Arens Sippy - University of Iowa, Microbiology and Immunology
- Resource Type
- Journal article
- Publication Details
- Molecular microbiology, Vol.122(4), pp.491-503
- Publisher
- Wiley
- DOI
- 10.1111/mmi.15306
- PMID
- 39233649
- ISSN
- 0950-382X
- eISSN
- 1365-2958
- Number of pages
- 13
- Grant note
- The Temperate Bacteriophage Fund, University of Iowa Foundation
- Language
- English
- Date published
- 10/2024
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984741057402771
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