DNA-RNA hybridization

J O Bishop; J S Beckmann; M S Campo; N D Hastie; M Izquierdo; S Perlman

doi:10.1098/rstb.1975.0077

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Journal article

Peer reviewed

DNA-RNA hybridization

J O Bishop, J S Beckmann, M S Campo, N D Hastie, M Izquierdo and S Perlman

Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol.272(915), pp.147-157

11/06/1975

DOI: 10.1098/rstb.1975.0077

PMID: 1811

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Abstract

Interest in nucleic acid hybridization stems mainly from its great power as a tool in biological research. It is used in several quite distinct ways. Because of the high degree of specificity that they show, hybridization techniques can be used to measure the amount of one specific sequence within a very heterogeneous mixture of sequences. Measurements of 1/10(6)-10(7) have been recorded. In extension of this, various properties of a specific sequence can often be studied. Secondly, because the kinetics of nucleic acid hybridization are quite well understood, it can be used to characterize both a pure sequence and a very complex mixture of sequences, like the genome of a vertebrate. Thirdly, again because of its specificity, it can be used to measure homologies between different populations of nucleic acids. Lastly, in conjunction with other techniques, it can be used as a basis for the fractionation of nucleic acid populations and the purification of specific sequences. Specific examples of these applications are given, with special reference to the organization of the genome in higher eukaryotes.

Nucleic Acid Hybridization

Genes

Cell Line

Rabbits

RNA Viruses - analysis

RNA, Messenger - analysis

RNA, Viral - analysis

Ducks

Poly A - analysis

Animals

DNA - analysis

Base Sequence

Chromosomes - analysis

Kinetics

Drosophila melanogaster

Hemoglobins

Details

Title: Subtitle: DNA-RNA hybridization
Creators: J O Bishop
J S Beckmann
M S Campo
N D Hastie
M Izquierdo
S Perlman
Resource Type: Journal article
Publication Details: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol.272(915), pp.147-157
DOI: 10.1098/rstb.1975.0077
PMID: 1811
NLM abbreviation: Philos Trans R Soc Lond B Biol Sci
ISSN: 0962-8436
eISSN: 1471-2970
Publisher: England
Language: English
Date published: 11/06/1975
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777356002771

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