DNA Replication but Not Nucleotide Excision Repair Is Required for UVC-Induced Replication Protein A Phosphorylation in Mammalian Cells

Gregory Rodrigo; Sophie Roumagnac; Marc S Wold; Bernard Salles; Patrick Calsou

doi:10.1128/MCB.20.8.2696-2705.2000

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DNA Replication but Not Nucleotide Excision Repair Is Required for UVC-Induced Replication Protein A Phosphorylation in Mammalian Cells

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DNA Replication but Not Nucleotide Excision Repair Is Required for UVC-Induced Replication Protein A Phosphorylation in Mammalian Cells

Gregory Rodrigo, Sophie Roumagnac, Marc S Wold, Bernard Salles and Patrick Calsou

Molecular and cellular biology, Vol.20(8), pp.2696-2705

04/2000

DOI: 10.1128/MCB.20.8.2696-2705.2000

PMCID: PMC85485

PMID: 10733572

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https://www.ncbi.nlm.nih.gov/pmc/articles/85485View

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Abstract

Exposure of mammalian cells to short-wavelength light (UVC) triggers a global response which can either counteract the deleterious effect of DNA damage by enabling DNA repair or lead to apoptosis. Several stress-activated protein kinases participate in this response, making phosphorylation a strong candidate for being involved in regulating the cellular damage response. One factor that is phosphorylated in a UVC-dependent manner is the 32-kDa subunit of the single-stranded DNA-binding replication protein A (RPA32). RPA is required for major cellular processes like DNA replication, and removal of DNA damage by nucleotide excision repair (NER). In this study we examined the signal which triggers RPA32 hyperphosphorylation following UVC irradiation in human cells. Hyperphosphorylation of RPA was observed in cells from patients with either NER or transcription-coupled repair (TCR) deficiency (A, C, and G complementation groups of xeroderma pigmentosum and A and B groups of Cockayne syndrome, respectively). This exclude both NER intermediates and TCR as essential signals for RPA hyperphosphorylation. However, we have observed that UV-sensitive cells deficient in NER and TCR require lower doses of UV irradiation to induce RPA32 hyperphosphorylation than normal cells, indicating that persistent unrepaired lesions contribute to RPA phosphorylation. Finally, the results of UVC irradiation experiments on nonreplicating cells and S-phase-synchronized cells emphasize a major role for DNA replication arrest in the presence of UVC lesions in RPA UVC-induced hyperphosphorylation in mammalian cells.

DNA Dynamics and Chromosome Structure

Details

Title: Subtitle: DNA Replication but Not Nucleotide Excision Repair Is Required for UVC-Induced Replication Protein A Phosphorylation in Mammalian Cells
Creators: Gregory Rodrigo - Institut de Pharmacologie et de Biologie Structurale, CNRS UPR 9062, F-31077 Toulouse Cedex, France
Sophie Roumagnac - Institut de Pharmacologie et de Biologie Structurale, CNRS UPR 9062, F-31077 Toulouse Cedex, France
Marc S Wold - Institut de Pharmacologie et de Biologie Structurale, CNRS UPR 9062, F-31077 Toulouse Cedex, France
Bernard Salles - Institut de Pharmacologie et de Biologie Structurale, CNRS UPR 9062, F-31077 Toulouse Cedex, France
Patrick Calsou - Institut de Pharmacologie et de Biologie Structurale, CNRS UPR 9062, F-31077 Toulouse Cedex, France
Resource Type: Journal article
Publication Details: Molecular and cellular biology, Vol.20(8), pp.2696-2705
DOI: 10.1128/MCB.20.8.2696-2705.2000
PMID: 10733572
PMCID: PMC85485
NLM abbreviation: Mol Cell Biol
ISSN: 0270-7306
eISSN: 1098-5549
Publisher: American Society for Microbiology
Language: English
Date published: 04/2000
Academic Unit: Radiation Oncology; Biochemistry and Molecular Biology
Record Identifier: 9984024544402771

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