DNA-binding activities of the Drosophila melanogaster even-skipped protein are mediated by its homeo domain and influenced by protein context

Timothy Hoey; Rahul Warrior; John Manak; Michael Levine

doi:10.1128/mcb.8.11.4598-4607.1988

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DNA-binding activities of the Drosophila melanogaster even-skipped protein are mediated by its homeo domain and influenced by protein context

Journal article

Peer reviewed

DNA-binding activities of the Drosophila melanogaster even-skipped protein are mediated by its homeo domain and influenced by protein context

Timothy Hoey, Rahul Warrior, John Manak and Michael Levine

Molecular and cellular biology, Vol.8(11), pp.4598-4607

11/1988

DOI: 10.1128/mcb.8.11.4598-4607.1988

PMCID: PMC365548

PMID: 2905420

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Abstract

The homeo box gene even-skipped (eve) encodes a 376-amino-acid protein that binds with high affinity to sequences located near the 5' termini of the eve and en genes. The 5' en sites are A + T rich and contain copies of the 10-base-pair (bp) consensus sequence T-C-A-A-T-T-A-A-A-T. In contrast, the 5' eve sites are G + C rich and contain the 9-bp sequence T-C-A-G-C-A-C-C-G. Among the five different homeo box proteins that have been tested for binding, eve is unique in that it shows virtually equal preference for the A + T-rich 5' en binding sites and the G + C-rich 5' eve sites. Most of the other proteins bind with a relatively higher affinity to the en sites than to the eve sites. In an effort to identify the regions of the eve protein that are responsible for its efficient binding to both classes of recognition sequences, we analyzed the DNA-binding properties of various mutant eve proteins. These studies suggest that the homeo domain of the eve protein is responsible for both binding activities. However, mutations in distant regions of the protein influenced the binding behavior of the eve homeo domain and caused a reduction in binding to the G + C class of recognition sites. We propose that the protein context of the homeo domain can influence its DNA-binding properties.

Mutation

Amino Acid Sequence

Drosophila Proteins

Molecular Sequence Data

DNA-Binding Proteins - genetics

Drosophila melanogaster - genetics

Bacterial Proteins

DNA-Binding Proteins - metabolism

Drosophila melanogaster - metabolism

Proteins - genetics

Animals

Proteins - metabolism

Homeodomain Proteins

Base Sequence

Transcription Factors

Binding Sites

Genes, Homeobox

Details

Title: Subtitle: DNA-binding activities of the Drosophila melanogaster even-skipped protein are mediated by its homeo domain and influenced by protein context
Creators: Timothy Hoey - Department of Biological Sciences, Columbia University, New York, New York 10027
Rahul Warrior
John Manak
Michael Levine
Resource Type: Journal article
Publication Details: Molecular and cellular biology, Vol.8(11), pp.4598-4607
DOI: 10.1128/mcb.8.11.4598-4607.1988
PMID: 2905420
PMCID: PMC365548
NLM abbreviation: Mol Cell Biol
ISSN: 0270-7306
eISSN: 1098-5549
Publisher: United States
Language: English
Date published: 11/1988
Academic Unit: Stead Family Department of Pediatrics; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9983992051302771

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