Journal article
DNA content analysis on microarrays
Methods in molecular biology (Clifton, N.J.), Vol.270, pp.237-248
2004
DOI: 10.1385/1-59259-793-9:237
PMID: 15153631
Abstract
The genome sequencing of protozoan parasites has facilitated the development of powerful postgenomics tools such as DNA microarrays and revolutionized the study of parasite biology. Large-scale genomic comparisons are useful in identifying the extent of genomic variability among related strains and isolates. Identification of deletions between geographically diverse clinical isolates is important in understanding parasite biology and the "fitness" of a given strain in dissemination. Additionally, the development of reliable diagnostic tests or identification of potential vaccine candidates is predicated on the large-scale conservation of the candidate genes. Parasites with variable virulence phenotypes (vaccine strain vs virulent strain) can also be studied for their genomic variability and provide further insights into the potential role of genotypic variability and its relationship to virulence. This chapter outlines the utilization of DNA microarrays to study genomic content.
Details
- Title: Subtitle
- DNA content analysis on microarrays
- Creators
- Upinder Singh - Stanford MedicinePreetam H. Shah - Stanford MedicineRyan C. MacFarlane - Stanford Medicine
- Resource Type
- Journal article
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.270, pp.237-248
- DOI
- 10.1385/1-59259-793-9:237
- PMID
- 15153631
- NLM abbreviation
- Methods Mol Biol
- ISSN
- 1064-3745
- Number of pages
- 12
- Language
- English
- Date published
- 2004
- Academic Unit
- Internal Medicine
- Record Identifier
- 9985244564502771
Metrics
1 Record Views