Journal article
DNA repair and cyclin D1 polymorphisms and styrene-induced genotoxicity and immunotoxicity
Toxicology and applied pharmacology, Vol.207(2), pp.302-309
2005
DOI: 10.1016/j.taap.2004.12.023
PMID: 15992842
Abstract
1-SO-adenine DNA adducts, DNA single-strand breaks (SBs), chromosomal aberrations (CAs), mutant frequency (MF) at the
HPRT gene, and immune parameters (hematological and of humoral immunity) were studied in styrene-exposed human subjects and controls. Results were correlated with genetic polymorphisms in DNA repair genes (
XPD, exon 23,
XPG, exon 15,
XPC, exon 15,
XRCC1, exon 10,
XRCC3, exon 7) and cell cycle gene
cyclin D1. Results for biomarkers of genotoxicity after stratification for the different DNA repair genetic polymorphisms showed that the polymorphism in exon 23 of the
XPD gene modulates levels of chromosomal and DNA damage,
HPRT MF, and moderately affects DNA adduct levels. The highest levels of biomarkers were associated with the wild-type homozygous
AA genotype. The exposed individuals with the wild-type
GG genotype for
XRCC1 gene exhibited the lowest CA frequencies, compared to those with an
A allele (
P < 0.05).
Cyclin D1 polymorphism seems to modulate the number of leukocytes and lymphocytes in the analyzed subjects. The number of eosinophiles was positively associated with
XPD variant
C allele and negatively with
XRCC1 variant
A allele (
P < 0.05) and
XPC variant
C allele (
P < 0.05). Immunoglobulin IgA was positively associated with an
XRCC3 variant
T allele (
P < 0.01) and negatively with
XPC variant
C allele (
P < 0.05). Both C3- and C4-complement components were lower in individuals with
XRCC3 CT (
P < 0.05) and
TT genotypes (
P < 0.01). Adhesion molecules sL-selectin and sICAM-1 were associated with
XPC genotype (
P < 0.05). Individual susceptibility may be reflected in genotoxic and immunotoxic responses to environmental and occupational exposures to xenobiotics.
Details
- Title: Subtitle
- DNA repair and cyclin D1 polymorphisms and styrene-induced genotoxicity and immunotoxicity
- Creators
- M. Kuricova - Czech Academy of SciencesA. Naccarati - Czech Academy of SciencesR. Kumar - German Cancer Research CenterM. Koskinen - Orion CorporationS. Sanyal - Karolinska InstitutetM. Dusinska - Slovak Medical UniversityJ. Tulinska - Slovak Medical UniversityL. Vodickova - Czech Academy of SciencesA. Liskova - Slovak Medical UniversityE. Jahnova - Slovak Medical UniversityL. Fuortes - University of IowaV. Haufroid - UCLouvainK. Hemminki - German Cancer Research CenterP. Vodicka - Czech Academy of Sciences
- Resource Type
- Journal article
- Publication Details
- Toxicology and applied pharmacology, Vol.207(2), pp.302-309
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.taap.2004.12.023
- PMID
- 15992842
- ISSN
- 0041-008X
- eISSN
- 1096-0333
- Language
- English
- Date published
- 2005
- Academic Unit
- Occupational and Environmental Health; Internal Medicine
- Record Identifier
- 9984363591402771
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