Journal article
DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice
Vaccine, Vol.35(16), pp.2069-2075
04/11/2017
DOI: 10.1016/j.vaccine.2017.02.063
PMCID: PMC5411280
PMID: 28314561
Abstract
•DNA vaccine encoding MERS-CoV S1 gene induced humoral and cellular immune responses.•High titers of neutralizing antibodies were generated without adjuvant.•Virus loads in lungs significantly decreased in vaccinated and serum received mice. The Middle East respiratory syndrome coronavirus (MERS-CoV), is an emerging pathogen that continues to cause outbreaks in the Arabian peninsula and in travelers from this region, raising the concern that a global pandemic could occur. Here, we show that a DNA vaccine encoding the first 725 amino acids (S1) of MERS-CoV spike (S) protein induces antigen-specific humoral and cellular immune responses in mice. With three immunizations, high titers of neutralizing antibodies (up to 1: 104) were generated without adjuvant. DNA vaccination with the MERS-CoV S1 gene markedly increased the frequencies of antigen-specific CD4+ and CD8+ T cells secreting IFN-γ and other cytokines. Both pcDNA3.1-S1 DNA vaccine immunization and passive transfer of immune serum from pcDNA3.1-S1 vaccinated mice protected Ad5-hDPP4-transduced mice from MERS-CoV challenge. These results demonstrate that a DNA vaccine encoding MERS-CoV S1 protein induces strong protective immune responses against MERS-CoV infection.
Details
- Title: Subtitle
- DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice
- Creators
- Hang Chi - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, ChinaXuexing Zheng - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, ChinaXiwen Wang - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, ChinaChong Wang - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, ChinaHualei Wang - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, ChinaWeiwei Gai - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, ChinaStanley Perlman - Department of Microbiology, University of Iowa, Iowa City, IA, USASongtao Yang - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, ChinaJincun Zhao - First Affiliated Hospital of Guangzhou Medical UniversityXianzhu Xia - Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China
- Resource Type
- Journal article
- Publication Details
- Vaccine, Vol.35(16), pp.2069-2075
- DOI
- 10.1016/j.vaccine.2017.02.063
- PMID
- 28314561
- PMCID
- PMC5411280
- NLM abbreviation
- Vaccine
- ISSN
- 0264-410X
- eISSN
- 1873-2518
- Publisher
- Elsevier Ltd
- Grant note
- DOI: 10.13039/100013262, name: State Key Laboratory of Respiratory Disease, award: 2014SKRD-001; name: Municipal Healthcare Joint-Innovation Major Project of Guangzhou, award: 201604020011; DOI: 10.13039/100000002, name: National Institutes of Health, award: RO1 AI091322, PO1 AI060699; name: National Science and Technology Pillar Program, award: 2013BAD12B04
- Language
- English
- Date published
- 04/11/2017
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
- Record Identifier
- 9983777350102771
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