Journal article
De-Differentiation of Mouse Interfollicular Keratinocytes by the Embryonic Transcription Factor Oct-4
Journal of Investigative Dermatology, Vol.127(2), pp.372-380
02/01/2007
DOI: 10.1038/sj.jid.5700531
Abstract
The embryonic transcription factor Oct-4 is often referred to as the master regulator of the undifferentiated state. Although its role in maintaining embryonic stem (ES) cell pluripotency is well established, its ability to directly reprogram committed somatic cells is not well defined. Using transient transfection, we tested its ability to revert mouse interfollicular epidermal basal keratinocytes to a more ES cell-like state. We found that the Oct-4-transfected keratinocytes expressed the Oct-4 target genes, Sox-2, Nanog, undifferentiated transcription factor 1 (Utf1), and Rex-1. We also noted an increase in developmental potential caused by Oct-4, with the transfected cells able to differentiate into neuronal cells when exposed to neuroectodermal differentiation medium. Control-transfected keratinocytes were unable to respond to the medium, and remained as keratinocytes. These findings suggest that Oct-4 may be the master regulator of the pluripotent state and demonstrate that differentiated somatic cells can be reverted into more developmentally potent cells through the use of a single factor. The latter finding has great implications for therapeutic cell-replacement applications using cells from easily accessible adult tissues, such as the skin.Journal of Investigative Dermatology (2007) 127, 372-380. doi:10.1038/sj.jid.5700531; published online 24 August 2006
Details
- Title: Subtitle
- De-Differentiation of Mouse Interfollicular Keratinocytes by the Embryonic Transcription Factor Oct-4
- Creators
- Katie L. GrinnellBaoli Yang - University of IowaRichard L. EckertJackie R. Bickenbach
- Resource Type
- Journal article
- Publication Details
- Journal of Investigative Dermatology, Vol.127(2), pp.372-380
- DOI
- 10.1038/sj.jid.5700531
- ISSN
- 0022-202X
- eISSN
- 1523-1747
- Language
- English
- Date published
- 02/01/2007
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9983557777402771
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