De novo recruitment of antigen-experienced and naive T cells contributes to the long-term maintenance of antiviral T cell populations in the persistently infected central nervous system

Jingxian Zhao; Jincun Zhao; Stanley Perlman

doi:10.4049/jimmunol.0902164

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De novo recruitment of antigen-experienced and naive T cells contributes to the long-term maintenance of antiviral T cell populations in the persistently infected central nervous system

Journal article

Peer reviewed

De novo recruitment of antigen-experienced and naive T cells contributes to the long-term maintenance of antiviral T cell populations in the persistently infected central nervous system

Jingxian Zhao, Jincun Zhao and Stanley Perlman

Journal of immunology (Baltimore, Md. : 1950), Vol.183(8), pp.5163-5170

10/15/2009

DOI: 10.4049/jimmunol.0902164

PMCID: PMC2811315

PMID: 19786545

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Abstract

Mice infected with attenuated strains of mouse hepatitis virus, strain JHM, develop a chronic infection in the brain and spinal cord characterized by low levels of viral Ag persistence and retention of virus-specific CD4 and CD8 T cells at the site of infection. It is not known whether these cells are maintained by proliferation of T cells that entered the CNS during acute infection or are newly recruited from Ag-experienced or naive T cell pools. In this study, using adoptive transfer experiments and bone marrow chimeras, we show that at least some of these cells are recruited from the periphery, predominantly from the viral Ag-experienced T cell pool. Both virus-specific CD4 and CD8 T cells are functional, as assessed by cytokine expression and degranulation after peptide exposure. In addition, populations of virus-specific CD4 T cells undergo dynamic changes in the infected CNS, as previously shown for CD8 T cells, because ratios of cells responding to two CD4 T cell epitopes change by a factor of five during the course of persistence. Collectively, these results show that maintenance of T cell responses in the virus-infected CNS is a dynamic process. Further, virus-specific T cell numbers at this site of infection are maintained by recruitment from peripheral Ag-experienced and naive T cell pools.

Antigens, Viral - metabolism

Mice, Inbred C57BL

Adoptive Transfer

Coronavirus Infections - immunology

Central Nervous System - immunology

Murine hepatitis virus

CD4-Positive T-Lymphocytes - immunology

Spinal Cord - immunology

Antigens, Viral - immunology

Animals

CD8-Positive T-Lymphocytes - virology

Mice

Spinal Cord - virology

CD4-Positive T-Lymphocytes - virology

CD8-Positive T-Lymphocytes - immunology

Central Nervous System Viral Diseases - immunology

Chronic Disease

Central Nervous System - virology

Details

Title: Subtitle: De novo recruitment of antigen-experienced and naive T cells contributes to the long-term maintenance of antiviral T cell populations in the persistently infected central nervous system
Creators: Jingxian Zhao - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Jincun Zhao
Stanley Perlman
Resource Type: Journal article
Publication Details: Journal of immunology (Baltimore, Md. : 1950), Vol.183(8), pp.5163-5170
DOI: 10.4049/jimmunol.0902164
PMID: 19786545
PMCID: PMC2811315
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: NS36592 / NINDS NIH HHS R01 NS036592-12 / NINDS NIH HHS R01 NS036592 / NINDS NIH HHS
Language: English
Date published: 10/15/2009
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777470302771

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