Deciliation is associated with dramatic remodeling of epithelial cell junctions and surface domains

Christian E Overgaard; Kaitlin M Sanzone; Krystle S Spiczka; David R Sheff; Alexander Sandra; Charles Yeaman

doi:10.1091/mbc.E08-07-0741

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Deciliation is associated with dramatic remodeling of epithelial cell junctions and surface domains

Journal article

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Peer reviewed

Deciliation is associated with dramatic remodeling of epithelial cell junctions and surface domains

Christian E Overgaard, Kaitlin M Sanzone, Krystle S Spiczka, David R Sheff, Alexander Sandra and Charles Yeaman

Molecular biology of the cell, Vol.20(1), pp.102-113

01/2009

DOI: 10.1091/mbc.E08-07-0741

PMCID: PMC2613083

PMID: 19005211

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Abstract

Stress-induced shedding of motile cilia (autotomy) has been documented in diverse organisms and likely represents a conserved cellular reaction. However, little is known about whether primary cilia are shed from mammalian epithelial cells and what impact deciliation has on polarized cellular organization. We show that several chemically distinct agents trigger autotomy in epithelial cells. Surprisingly, deciliation is associated with a significant, but reversible increase in transepithelial resistance. This reflects substantial reductions in tight junction proteins associated with "leaky" nephron segments (e.g., claudin-2). At the same time, apical trafficking of gp80/clusterin and gp114/CEACAM becomes randomized, basal-lateral delivery of Na,K-ATPase is reduced, and expression of the nonciliary apical protein gp135/podocalyxin is greatly decreased. However, ciliogenesis-impaired MDCK cells do not undergo continual junction remodeling, and mature cilia are not required for autotomy-associated remodeling events. Deciliation and epithelial remodeling may be mechanistically linked processes, because RNAi-mediated reduction of Exocyst subunit Sec6 inhibits ciliary shedding and specifically blocks deciliation-associated down-regulation of claudin-2 and gp135. We propose that ciliary autotomy represents a signaling pathway that impacts the organization and function of polarized epithelial cells.

Tight Junctions - metabolism

Cell Line

Cell Polarity

Epithelial Cells - metabolism

Membrane Glycoproteins - metabolism

Calcium - metabolism

Sialoglycoproteins - metabolism

Cell Adhesion Molecules - metabolism

Cilia - metabolism

Antigens, CD - metabolism

Clusterin - metabolism

Animals

Dogs

Signal Transduction - physiology

Biological Transport - physiology

Epithelial Cells - cytology

Details

Title: Subtitle: Deciliation is associated with dramatic remodeling of epithelial cell junctions and surface domains
Creators: Christian E Overgaard - Department of Anatomy, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Kaitlin M Sanzone
Krystle S Spiczka
David R Sheff
Alexander Sandra
Charles Yeaman
Resource Type: Journal article
Publication Details: Molecular biology of the cell, Vol.20(1), pp.102-113
DOI: 10.1091/mbc.E08-07-0741
PMID: 19005211
PMCID: PMC2613083
NLM abbreviation: Mol Biol Cell
ISSN: 1059-1524
eISSN: 1939-4586
Publisher: American Society for Cell Biology; United States
Grant note: P50 DK052617 / NIDDK NIH HHS DK052617 / NIDDK NIH HHS R01 GM067002 / NIGMS NIH HHS GM067002 / NIGMS NIH HHS
Language: English
Date published: 01/2009
Academic Unit: Anatomy and Cell Biology; Family and Community Medicine; Internal Medicine
Record Identifier: 9984025314402771

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