Deciphering the DNA code for the function of the Drosophila polydactyl zinc finger protein Suppressor of Hairy-wing

Ryan M Baxley; James D Bullard; Michael W Klein; Ashley G Fell; Joel A Morales-Rosado; Tingting Duan; Pamela K Geyer

doi:10.1093/nar/gkx040

Back

Deciphering the DNA code for the function of the Drosophila polydactyl zinc finger protein Suppressor of Hairy-wing

Journal article

Open access

Peer reviewed

Deciphering the DNA code for the function of the Drosophila polydactyl zinc finger protein Suppressor of Hairy-wing

Ryan M Baxley, James D Bullard, Michael W Klein, Ashley G Fell, Joel A Morales-Rosado, Tingting Duan and Pamela K Geyer

Nucleic acids research, Vol.45(8), pp.4463-4478

05/05/2017

DOI: 10.1093/nar/gkx040

PMCID: PMC5416891

PMID: 28158673

Files and links (1)

url

https://doi.org/10.1093/nar/gkx040View

Published (Version of record) Open Access

Abstract

Polydactyl zinc finger (ZF) proteins have prominent roles in gene regulation and often execute multiple regulatory functions. To understand how these proteins perform varied regulation, we studiedDrosophila Suppressor of Hairy-wing [Su(Hw)], an exemplar multifunctional polydactyl ZF protein. We identified separation-of-function (SOF) alleles that encode proteins disrupted in a single ZF that retain one of the Su(Hw) regulatory activities. Through extended in vitro analyses of the Su(Hw) ZF domain, we show that clusters of ZFs bind individual modules within a compound DNA consensus sequence. Through in vivo analysis of SOF mutants, we find that Su(Hw) genomic sites separate into sequence subclasses comprised of combinations of modules, with subclasses enriched for different chromatin features. These data suggest a Su(Hw) code, wherein DNA binding dictates its cofactor recruitment and regulatory output. We propose that similar DNA codes might be used to confer multiple regulatory functions of other polydactyl ZF proteins.

Chromatin - metabolism

Male

Drosophila Proteins - metabolism

Drosophila melanogaster - genetics

Drosophila melanogaster - metabolism

Base Sequence

Protein Domains

Female

Binding Sites

Chromatin - drug effects

Repressor Proteins - metabolism

Chromatin - chemistry

Zinc Fingers

Gene Expression Regulation

Mutagens - pharmacology

Repressor Proteins - genetics

Genotype

DNA - metabolism

Drosophila melanogaster - drug effects

DNA - genetics

Phenotype

Animals

Ethyl Methanesulfonate - pharmacology

Alleles

Protein Binding

Drosophila Proteins - genetics

Mutation

Details

Title: Subtitle: Deciphering the DNA code for the function of the Drosophila polydactyl zinc finger protein Suppressor of Hairy-wing
Creators: Ryan M Baxley - Interdisciplinary Graduate Program in Molecular and Cellular Biology, University of Iowa, Iowa City, IA 52242, USA
James D Bullard - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Michael W Klein - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Ashley G Fell - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Joel A Morales-Rosado - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Tingting Duan - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Pamela K Geyer - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Nucleic acids research, Vol.45(8), pp.4463-4478
Publisher: England
DOI: 10.1093/nar/gkx040
PMID: 28158673
PMCID: PMC5416891
ISSN: 0305-1048
eISSN: 1362-4962
Grant note: R01 GM042539 / NIGMS NIH HHS P30 CA086862 / NCI NIH HHS
Language: English
Date published: 05/05/2017
Academic Unit: Obstetrics and Gynecology; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984025285602771

Metrics

28 Record Views

26 Times Cited - Web of Science