Decoding Polo-like kinase 1 signaling along the kinetochore-centromere axis

Robert F Lera; Gregory K Potts; Aussie Suzuki; James M Johnson; Edward D Salmon; Joshua J Coon; Mark E Burkard

doi:10.1038/nchembio.2060

Back

Decoding Polo-like kinase 1 signaling along the kinetochore-centromere axis

Journal article

Peer reviewed

Decoding Polo-like kinase 1 signaling along the kinetochore-centromere axis

Robert F Lera, Gregory K Potts, Aussie Suzuki, James M Johnson, Edward D Salmon, Joshua J Coon and Mark E Burkard

Nature chemical biology, Vol.12(6), pp.411-418

06/01/2016

DOI: 10.1038/nchembio.2060

PMCID: PMC4871769

PMID: 27043190

View Online

Abstract

Protein kinase signaling along the kinetochore-centromere axis is crucial to assure mitotic fidelity, yet the details of its spatial coordination are obscure. Here, we examined how pools of human Polo-like kinase 1 (Plk1) within this axis control signaling events to elicit mitotic functions. To do this, we restricted active Plk1 to discrete subcompartments within the kinetochore-centromere axis using chemical genetics and decoded functional and phosphoproteomic signatures of each. We observe distinct phosphoproteomic and functional roles, suggesting that Plk1 exists and functions in discrete pools along this axis. Deep within the centromere, Plk1 operates to assure proper chromosome alignment and segregation. Thus, Plk1 at the kinetochore is a conglomerate of an observable bulk pool coupled with additional functional pools below the threshold of microscopic detection or resolution. Although complex, this multiplicity of locales provides an opportunity to decouple functional and phosphoproteomic signatures for a comprehensive understanding of Plk1's kinetochore functions.

Cell Cycle Proteins - metabolism

Centromere - metabolism

Humans

Kinetochores - metabolism

Polo-Like Kinase 1

Protein Serine-Threonine Kinases - metabolism

Proto-Oncogene Proteins - metabolism

Signal Transduction

Details

Title: Subtitle: Decoding Polo-like kinase 1 signaling along the kinetochore-centromere axis
Creators: Robert F Lera - University of Wisconsin Carbone Cancer Center
Gregory K Potts - University of Wisconsin–Madison
Aussie Suzuki - Department of Biology, University of North Carolina, Chapel Hill, North Carolina, USA
James M Johnson - University of Wisconsin Carbone Cancer Center
Edward D Salmon - Department of Biology, University of North Carolina, Chapel Hill, North Carolina, USA
Joshua J Coon - University of Wisconsin–Madison
Mark E Burkard - University of Wisconsin Carbone Cancer Center
Resource Type: Journal article
Publication Details: Nature chemical biology, Vol.12(6), pp.411-418
DOI: 10.1038/nchembio.2060
PMID: 27043190
PMCID: PMC4871769
ISSN: 1552-4450
eISSN: 1552-4469
Grant note: R01 GM080148 / NIGMS NIH HHS R01 GM097245 / NIGMS NIH HHS P30 CA016086 / NCI NIH HHS R01 GM024364 / NIGMS NIH HHS P30 CA014520 / NCI NIH HHS
Language: English
Date published: 06/01/2016
Academic Unit: Internal Medicine
Record Identifier: 9984700652602771

Metrics

5 Record Views

36 Times Cited - Web of Science

See more details