Journal article
Decreased myo-inositol uptake is associated with reduced bradykinin-stimulated phosphatidylinositol synthesis and diacylglycerol content in cultured neuroblastoma cells exposed to L-fucose
Journal of neurochemistry, Vol.62(1), pp.147-158
1994
DOI: 10.1046/j.1471-4159.1994.62010147.x
PMID: 8263514
Abstract
L-Fucose is a potent, competitive inhibitor of myo-inositol transport by cultured mammalian cells. Chronic exposure of neuroblastoma cells to L-fucose causes a concentration-dependent decrease in myo-inositol content, accumulation, and incorporation into phosphoinositides. In these studies, L-fucose supplementation of culture medium was used to assess the effect of decreased myo-inositol metabolism and content on bradykinin-stimulated phosphatidylinositol synthesis and diacylglycerol production. Chronic exposure of cells to 30 mML-fucose caused a sustained decrease in bradykinin-stimulated, but not basal, 3H-inositol phosphate release and 32P incorporation into phosphatidylinositol in cells incubated in serum-free, unsupplemented medium. In addition, 32P incorporation into phosphatidylinositol 4-phosphate and phosphatidylinositol 4, 5-bisphosphate was not altered in L-fucose-conditioned cells. Acute exposure of cells to serum-free medium containing 30 mM L-fucose did not affect either basal or bradykinin-stimulated 32P incorporation into phosphatidylinositol. Basal diacylglycerol content was decreased by 20% in cells chronically exposed to 30 mM L-fucose, although analysis of the molecular species profile revealed no compositional change. Bradykinin stimulated diacylglycerol production in neuroblastoma cells by increasing the hydrolysis of both phosphoinositides and phosphatidylcholine. Bradykinin-stimulated production of total diacylglycerol was similar for control and L-fucose-conditioned cells. However, there was a decrease in the bradykinin-induced generation of the 1 -stearoyl-2-arachidonoyl diacylglycerol molecular species in the cells chronically exposed to 30 mM L-fucose. This molecular species accounts for about 70% of the composition of phosphoinositides, but only 10% of phosphatidylcholine. The results suggest that a decrease in myo-inositol uptake results in diminished agonist-induced phosphatidylinositol synthesis and phosphoinositide hydrolysis in cultured neuroblastoma cells grown in L-fucose-containing medium.
Details
- Title: Subtitle
- Decreased myo-inositol uptake is associated with reduced bradykinin-stimulated phosphatidylinositol synthesis and diacylglycerol content in cultured neuroblastoma cells exposed to L-fucose
- Creators
- Mark A Yorek - Veterans administration medical cent., Iowa City IA 52245, United StatesJoyce A Dunlap - Veterans administration medical cent., Iowa City IA 52245, United StatesMark R Stefani - Veterans administration medical cent., Iowa City IA 52245, United StatesEric P Davidson - Veterans administration medical cent., Iowa City IA 52245, United StatesXi Zhu - Veterans administration medical cent., Iowa City IA 52245, United StatesJoseph Eichberg - Veterans administration medical cent., Iowa City IA 52245, United States
- Resource Type
- Journal article
- Publication Details
- Journal of neurochemistry, Vol.62(1), pp.147-158
- Publisher
- Blackwell
- DOI
- 10.1046/j.1471-4159.1994.62010147.x
- PMID
- 8263514
- ISSN
- 0022-3042
- eISSN
- 1471-4159
- Language
- English
- Date published
- 1994
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094587702771
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