Decreased norepinephrine in the ventral lamina terminalis region is associated with angiotensin II drinking response deficits following local 6-hydroxydopamine injections

J T Cunningham; A K Johnson

doi:10.1016/0006-8993(89)91567-9

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Decreased norepinephrine in the ventral lamina terminalis region is associated with angiotensin II drinking response deficits following local 6-hydroxydopamine injections

Journal article

Peer reviewed

Decreased norepinephrine in the ventral lamina terminalis region is associated with angiotensin II drinking response deficits following local 6-hydroxydopamine injections

J T Cunningham and A K Johnson

Brain research, Vol.480(1-2), pp.65-71

02/20/1989

DOI: 10.1016/0006-8993(89)91567-9

PMID: 2496890

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Abstract

Previous studies have demonstrated that punctate injections of 6-hydroxydopamine (6-OHDA) into structures along the ventral lamina terminalis reduce drinking responses elicited by angiotensin II (ANG II). The purpose of the present study was to extend these findings by quantifying the catecholamine depletions that are associated with the drinking response deficits. Male Sprague-Dawley rats were given injections of 6-OHDA into the median preoptic nucleus (MnPO) and the organum vasculosum of the lamina terminalis (OVLT) after pretreatment with pargyline. Controls were injected with vehicle and a third group received 6-OHDA after pretreatment with desmethylimipramine (DMI). All subjects were tested for drinking responses to centrally injected ANG II and carbachol. Changes in catecholamine content of the MnPO, OVLT, supraoptic nucleus, paraventricular nucleus, caudate and cortex were determined using HPLC with electrochemical detection after behavioral testing. Only rats injected with 6-OHDA without DMI pretreatment showed a significant reduction in ANG II-induced drinking responses. This behavioral deficit was associated with a significant norepinephrine decrease in the ventral lamina terminalis region. All 3 groups showed comparable drinking responses to carbachol. These data support the hypothesis that noradrenergic innervation of the ventral lamina terminalis region is necessary for ANG II-induced drinking responses.

Angiotensin II - pharmacology

Animals

Carbachol - pharmacology

Dose-Response Relationship, Drug

Drinking Behavior - drug effects

Hydroxydopamines

Hypothalamus - drug effects

Hypothalamus - metabolism

Hypothalamus - physiology

Male

Microinjections

Norepinephrine - metabolism

Norepinephrine - physiology

Oxidopamine

Rats

Rats, Inbred Strains

Details

Title: Subtitle: Decreased norepinephrine in the ventral lamina terminalis region is associated with angiotensin II drinking response deficits following local 6-hydroxydopamine injections
Creators: J T Cunningham - Department of Psychology, University of Iowa, Iowa City 52242
A K Johnson
Resource Type: Journal article
Publication Details: Brain research, Vol.480(1-2), pp.65-71
DOI: 10.1016/0006-8993(89)91567-9
PMID: 2496890
ISSN: 0006-8993
eISSN: 1872-6240
Grant note: HL33447 / NHLBI NIH HHS HL14558 / NHLBI NIH HHS HL33796 / NHLBI NIH HHS
Language: English
Date published: 02/20/1989
Academic Unit: Psychological and Brain Sciences; Neuroscience and Pharmacology; Health and Human Physiology
Record Identifier: 9984213407802771

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