Decreasing Bacterial Colonization of External Fixation Pins Through Nitric Oxide Release Coatings

Joshua HOLT; Brian HERTZBERG; Paul WEINHOLD; Wesley STORM; Mark SCHOENFISCH; Laurence DAHNERS

doi:10.1097/BOT.0b013e3181f9ac8a

Back

Decreasing Bacterial Colonization of External Fixation Pins Through Nitric Oxide Release Coatings

Journal article

Open access

Peer reviewed

Decreasing Bacterial Colonization of External Fixation Pins Through Nitric Oxide Release Coatings

Joshua HOLT, Brian HERTZBERG, Paul WEINHOLD, Wesley STORM, Mark SCHOENFISCH and Laurence DAHNERS

Journal of orthopaedic trauma, Vol.25(7), pp.432-437

2011

DOI: 10.1097/BOT.0b013e3181f9ac8a

PMID: 21637124

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/3117952View

Open Access

Abstract

Objective: Bacterial infection of the pin tract represents the most common complication associated with external fixation. This study was designed to evaluate the antibacterial activity of nitric oxide (NO)-releasing xerogel films applied to commercially pure titanium pins in a rat model. Methods: Pins were coated with xerogel solution through a dip-coating procedure. Half of the xerogel-coated implant pins were modified into NO donors and served as the NO-releasing group, whereas the remaining pins were left unmodified to serve as non-NO-releasing xerogel-coated controls. Acid-etched pins served as uncoated controls. Animal selection was randomized and every rat had one pin from each of the three groups randomly allocated to the third, fourth, or fifth tail vertebrae. Quantification of bacterial infection was performed 48 days postoperatively and the tissue-implant interface was inspected for clinical signs of infection on Days 14 and 28 postimplantation. Results: Pin tract bacterial colony counts of the NO-releasing group (170,000 ± 181,000) were significantly lower than both the xerogel-coated group (677,000 ± 675,000) and the control group (1,181,000 ± 2,717,000) 48 days postoperatively (P < 0.05). No significant difference in colony counts was observed between the xerogel-coated group and the control group. The NO-releasing group also had significantly fewer clinical signs of infection than both the coated and the control groups on postoperative Day 28 (P < 0.05). Conclusion: The application of NO-releasing xerogel coatings can inhibit bacterial colonization of external fixation pins both during the initial postsurgical period and up to 48 days postimplantation.

Biological and medical sciences

Medical sciences

Antibacterial agents

Pharmacology. Drug treatments

Diseases of the osteoarticular system

Antibiotics. Antiinfectious agents. Antiparasitic agents

Details

Title: Subtitle: Decreasing Bacterial Colonization of External Fixation Pins Through Nitric Oxide Release Coatings
Creators: Joshua HOLT - University of North Carolina School of Medicine, Chapel Hill, NC, United States
Brian HERTZBERG - University of North Carolina School of Medicine, Chapel Hill, NC, United States
Paul WEINHOLD - Department of Orthopaedics, University of North Carolina School of Medicine, Chapel Hill, NC, United States
Wesley STORM - Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States
Mark SCHOENFISCH - Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States
Laurence DAHNERS - Department of Orthopaedics, University of North Carolina School of Medicine, Chapel Hill, NC, United States
Resource Type: Journal article
Publication Details: Journal of orthopaedic trauma, Vol.25(7), pp.432-437
DOI: 10.1097/BOT.0b013e3181f9ac8a
PMID: 21637124
NLM abbreviation: J Orthop Trauma
ISSN: 0890-5339
eISSN: 1531-2291
Publisher: Lippincott Williams & Wilkins; Hagerstown, MD
Language: English
Date published: 2011
Academic Unit: Stead Family Department of Pediatrics; Orthopedics and Rehabilitation
Record Identifier: 9984040225402771

Metrics

27 Record Views

44 Times Cited - Web of Science