Deep tissue incision enhances spinal dorsal horn neuron activity during static isometric muscle contraction in rats

He Gu; Daisuke Sugiyama; Sinyoung Kang; Timothy J Brennan

doi:10.1016/j.jpain.2018.09.012

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Deep tissue incision enhances spinal dorsal horn neuron activity during static isometric muscle contraction in rats

Journal article

Open access

Peer reviewed

Deep tissue incision enhances spinal dorsal horn neuron activity during static isometric muscle contraction in rats

He Gu, Daisuke Sugiyama, Sinyoung Kang and Timothy J Brennan

The journal of pain, Vol.20(3), pp.301-314

03/2019

DOI: 10.1016/j.jpain.2018.09.012

PMCID: PMC6382557

PMID: 30296612

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Abstract

Translational correlates to pain with activities after deep tissue injury have not been studied to a great extent. We hypothesized that deep tissue incision causes greater activation of nociception-transmitting neurons evoked by muscle contraction. In vivo neuronal activity was recorded in 203 dorsal horn neurons (DHNs) from 97 rats after sham, skin-only, or skin + deep muscle incision. We evaluated DHN responses to static, isometric muscle contractions induced by direct electrical stimulation of the muscle. The effect of pancuronium on DHN response to contractions was also examined. Approximately 50% of DHNs with receptive fields in the hindpaw was excited during muscle contraction. One-second, 0.5- and 1.0-gram muscle contractions produced greater DHN activity after skin + deep muscle incision [median (interquartile range): 32 (5 – 39); p = 0.021 and 36 (26 – 46) impulses; p = 0.006, respectively] than after sham [6 (0 – 21) and 15 (8 – 32) impulses, respectively]. Neuromuscular blockade with pancuronium inhibited the muscle contractions and DHN activation during electrical stimulation, demonstrating contraction-induced activation. The greater response of spinal DHNs to static muscle contraction after skin + deep muscle incision may model and inform mechanisms of dynamic pain after surgery.

in vivo extracellular recordings

static isometric muscle contraction

spinal dorsal horn neuron activity

postoperative pain

Deep tissue incision

Details

Title: Subtitle: Deep tissue incision enhances spinal dorsal horn neuron activity during static isometric muscle contraction in rats
Creators: He Gu - Department of Anesthesia, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa 52241, USA
Daisuke Sugiyama - Department of Anesthesia, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa 52241, USA
Sinyoung Kang - Department of Anesthesia, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa 52241, USA
Timothy J Brennan - Department of Anesthesia, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa 52241, USA
Resource Type: Journal article
Publication Details: The journal of pain, Vol.20(3), pp.301-314
DOI: 10.1016/j.jpain.2018.09.012
PMID: 30296612
PMCID: PMC6382557
NLM abbreviation: J Pain
ISSN: 1526-5900
eISSN: 1528-8447
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health, award: GM067762, ., GM124055
Language: English
Date published: 03/2019
Academic Unit: Anesthesia
Record Identifier: 9984007163002771

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