Journal article
Defective Break-Induced Replication Leads to Half-Crossovers in Saccharomyces cerevisiae
Genetics (Austin), Vol.179(4), pp.1845-1860
08/2008
DOI: 10.1534/genetics.108.087940
PMCID: PMC2516063
PMID: 18689895
Abstract
Break-induced replication (BIR) is an important process of DNA metabolism that has been implicated in the restart of collapsed replication forks, as well as in various chromosomal instabilities, including loss of heterozygosity, translocations, and alternative telomere lengthening. Therefore, knowledge of how BIR is carried out and regulated is important for better understanding the maintenance of genomic stability in eukaryotes. Here we present a new yeast experimental system that enables the genetic control of BIR to be investigated. Analysis of mutations selected on the basis of their sensitivity to various DNA-damaging agents demonstrated that deletion of
POL32
, which encodes a third, nonessential subunit of polymerase δ, significantly reduced the efficiency of BIR, although some
POL32
-independent BIR was still observed. Importantly, the BIR defect in
pol32
Δ cells was associated with the formation of half-crossovers. We propose that these half-crossovers resulted from aberrant processing of BIR intermediates. Furthermore, we suggest that the half-crossovers observed in our system are analogous to nonreciprocal translocations (NRTs) described in mammalian tumor cells and, thus, our system could represent an opportunity to further study the NRT mechanism in yeast.
Details
- Title: Subtitle
- Defective Break-Induced Replication Leads to Half-Crossovers in Saccharomyces cerevisiae
- Creators
- Angela Deem - Department of Biology, School of Science, Indiana University–Purdue University Indianapolis, Indianapolis, Indiana 46202-5132Krista Barker - Department of Biology, School of Science, Indiana University–Purdue University Indianapolis, Indianapolis, Indiana 46202-5132Kelly VanHulle - Department of Biology, School of Science, Indiana University–Purdue University Indianapolis, Indianapolis, Indiana 46202-5132Brandon Downing - Department of Biology, School of Science, Indiana University–Purdue University Indianapolis, Indianapolis, Indiana 46202-5132Alexandra Vayl - Department of Biology, School of Science, Indiana University–Purdue University Indianapolis, Indianapolis, Indiana 46202-5132Anna Malkova - Department of Biology, School of Science, Indiana University–Purdue University Indianapolis, Indianapolis, Indiana 46202-5132
- Resource Type
- Journal article
- Publication Details
- Genetics (Austin), Vol.179(4), pp.1845-1860
- DOI
- 10.1534/genetics.108.087940
- PMID
- 18689895
- PMCID
- PMC2516063
- NLM abbreviation
- Genetics
- ISSN
- 0016-6731
- eISSN
- 1943-2631
- Publisher
- Genetics Society of America
- Language
- English
- Date published
- 08/2008
- Academic Unit
- Biology
- Record Identifier
- 9984217423502771
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