Journal article
Defective CD3-Mediated Cell Death in Activated T Cells from Patients with Systemic Lupus Erythematosus: Role of Decreased Intracellular TNF-α
Clinical immunology and immunopathology, Vol.81(3), pp.293-302
12/1996
DOI: 10.1006/clin.1996.0192
PMID: 8938108
Abstract
Activation-induced cell death (AICD) plays an important role in the regulation of the immune response by eliminating preactivated and potentially autoreactive cells. To elucidate possible abnormalities of AICD in human systemic lupus erythematosus (SLE), we studied AICD in activated T cells from patients with SLE and normal controls. CD3-mediated cell death was determined in short-term T cell lines by flow cytometry using propidium iodide staining and analysis of DNA subdiploid peak populations. It was found to be significantly lower in T cells from SLE patients compared to cells from normal controls. Anti-Fas mAb-mediated cell death was similar in SLE and control cell lines. CD3-mediated AICD could be blocked in control and SLE T cell lines by an IgG anti-Fas mAb. Indirect immunofluorescence analysis showed statistically significantly less intracellular TNF-α in SLE T cells than in control cells. These data show that activated T cells from patients with SLE are relatively resistant to a TCR-mediated death stimulus although they display intact anti-Fas mAb-mediated cell death. Defective antigen-mediated cell death can contribute to increased numbers of activated autoreactive cells in lupus patients.
Details
- Title: Subtitle
- Defective CD3-Mediated Cell Death in Activated T Cells from Patients with Systemic Lupus Erythematosus: Role of Decreased Intracellular TNF-α
- Creators
- Birgit Kovacs - Walter Reed Army Institute of ResearchDimitrios Vassilopoulos - Walter Reed Army Institute of ResearchScott A. Vogelgesang - Walter Reed Army Medical CenterGeorge C. Tsokos - Walter Reed Army Institute of Research
- Resource Type
- Journal article
- Publication Details
- Clinical immunology and immunopathology, Vol.81(3), pp.293-302
- Publisher
- Elsevier Inc
- DOI
- 10.1006/clin.1996.0192
- PMID
- 8938108
- ISSN
- 0090-1229
- eISSN
- 1090-2341
- Language
- English
- Date published
- 12/1996
- Academic Unit
- Immunology; Internal Medicine; Ophthalmology and Visual Sciences
- Record Identifier
- 9984360151502771
Metrics
11 Record Views