Journal article
Defensin-induced adaptive immunity in mice and its potential in preventing periodontal disease
Oral microbiology and immunology, Vol.18(2), pp.95-99
2003
DOI: 10.1034/j.1399-302X.2003.00047.x
PMID: 12654098
Abstract
The severity of periodontal disease is dependent on a combination of host, microbial agent and environmental factors. One strong correlate related to periodontal disease pathogenesis is the immune status of the host. Here we show that human neutrophil peptide (HNP) defensins or human beta-defensins (HBD), co-administered intranasally with the antigen ovalbumin (OVA), induce unique immune responses that if used with microbial antigens may have the potential to hinder the pathogenesis of periodontal disease. C57BL/6 mice were immunized intranasally with phosphate buffered saline (PBS) containing 1 micro g HNP-1, HNP-2, HBD1 or HBD2 with and without 50 microg OVA. At 21 days, isotypes and subclasses of OVA-specific antibodies were determined in saliva, serum, nasal wash, bronchoalveolar lavage fluid, and fecal extracts. OVA-stimulated splenic lymphoid cell cultures from immunized mice were assessed for interferon (IFN)-gamma, Interleukin (IL)-4 and IL-10. In comparison with mice immunized with only OVA, HNP-1 and HBD2 induced significantly higher (P < 0.05) OVA-specific serum IgG, lower, but not significant, serum IgM and significantly lower (P < 0.05) IFN-gamma. In contrast, HNP-2 induced low OVA-specific serum IgG and higher, but not significant, serum IgM. HBD1 induced significantly higher (P < 0.05) OVA-specific serum IgG, higher, but not significant, serum IgM, and significantly higher (P < 0.05) IL-10. The elevated serum IgG subclasses contained IgG1 and IgG2b.
Details
- Title: Subtitle
- Defensin-induced adaptive immunity in mice and its potential in preventing periodontal disease
- Creators
- K. A BROGDEN - Respiratory Diseases of Livestock Research Unit, USDA, Agricultural Research Service, National Animal Disease Center, Ames, IA, United StatesM HEIDARI - Respiratory Diseases of Livestock Research Unit, USDA, Agricultural Research Service, National Animal Disease Center, Ames, IA, United StatesR. E SACCO - Respiratory Diseases of Livestock Research Unit, USDA, Agricultural Research Service, National Animal Disease Center, Ames, IA, United StatesD PALMQUIST - USDA, Agricultural Research Service, Peoria, IL, United StatesJ. M GUTHMILLER - Department of Periodontics and Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA, United StatesG. K JOHNSON - Department of Periodontics and Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA, United StatesH. P JIA - Department of Pediatrics, College of Medicine, The University of Iowa, Iowa City, IA, United StatesB. F TACK - Department of Microbiology, College of Medicine, The University of Iowa, Iowa City, IA, United StatesP. B MCCRAY - Department of Pediatrics, College of Medicine, The University of Iowa, Iowa City, IA, United States
- Resource Type
- Journal article
- Publication Details
- Oral microbiology and immunology, Vol.18(2), pp.95-99
- Publisher
- Blackwell; Oxford
- DOI
- 10.1034/j.1399-302X.2003.00047.x
- PMID
- 12654098
- ISSN
- 0902-0055
- eISSN
- 1399-302X
- Language
- English
- Date published
- 2003
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Periodontics; Internal Medicine
- Record Identifier
- 9984065713602771
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