Delays in GABAergic interneuron development and behavioral inhibition after prenatal stress

Stephanie J Lussier; Hanna E Stevens

doi:10.1002/dneu.22376

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Delays in GABAergic interneuron development and behavioral inhibition after prenatal stress

Journal article

Open access

Peer reviewed

Delays in GABAergic interneuron development and behavioral inhibition after prenatal stress

Stephanie J Lussier and Hanna E Stevens

Developmental neurobiology (Hoboken, N.J.), Vol.76(10), pp.1078-1091

10/2016

DOI: 10.1002/dneu.22376

PMCID: PMC6355144

PMID: 26724783

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https://www.ncbi.nlm.nih.gov/pmc/articles/6355144View

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Abstract

Prenatal stress is associated with altered behavioral, cognitive, and psychiatric outcomes in offspring. Due to the importance of GABAergic systems in normal development and in psychiatric disorders, prenatal stress effects on these neurons have been investigated in animal models. Prenatal stress delays GABAergic progenitor migration, but the significance of these early developmental disruptions for the continued development of GABAergic cells in the juvenile brain is unclear. Here, we examined effects of prenatal stress on populations of GABAergic neurons in juvenile and adult medial frontal cortex (mFC) and hippocampus through stereological counting, gene expression, and relevant anxiety-like and social behaviors. Postnatally, the total GABAergic cell number that peaks in adolescence showed altered trajectories in mFC and hippocampus. Parvalbumin neuron proportion in juvenile brain was altered by prenatal stress, but parvalbumin gene expression showed no differences. In adult brain, parvalbumin neuron proportions were altered by prenatal stress with opposite gene expression changes. Adult prenatally stressed offspring showed a lack of social preference on a three-chambered task, increased anxiety-like behavior on the elevated plus maze, and reduced center time in an open field. Despite a lack of significant group differences in adult total GABAergic cell populations, performance of these tasks was correlated with GABAergic populations in mFC and hippocampus. In conclusion, prenatal stress resulted in a delay in GABAergic cell number and maturation of the parvalbumin subtype. Influences of prenatal stress on GABAergic populations during developmentally dynamic periods and during adulthood may be relevant to the anxiety-like behaviors that occur after prenatal stress. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 1078-1091, 2016.

Immunohistochemistry

Green Fluorescent Proteins - metabolism

Frontal Lobe - metabolism

Cell Count

Parvalbumins - metabolism

Glutamate Decarboxylase - genetics

Male

Mice, Transgenic

Green Fluorescent Proteins - genetics

Prenatal Exposure Delayed Effects - metabolism

Pregnancy

Hippocampus - metabolism

Animals

Interneurons - metabolism

Gene Expression Regulation, Developmental

Stress, Psychological - metabolism

Polymerase Chain Reaction

Frontal Lobe - growth & development

Glutamate Decarboxylase - metabolism

Female

GABAergic Neurons - metabolism

Hippocampus - growth & development

Details

Title: Subtitle: Delays in GABAergic interneuron development and behavioral inhibition after prenatal stress
Creators: Stephanie J Lussier - Department of Psychiatry, University of Iowa Carver College of Medicine, Iowa City, IA
Hanna E Stevens - Yale Child Study Center, Yale School of Medicine, New Haven, Connecticut
Resource Type: Journal article
Publication Details: Developmental neurobiology (Hoboken, N.J.), Vol.76(10), pp.1078-1091
DOI: 10.1002/dneu.22376
PMID: 26724783
PMCID: PMC6355144
NLM abbreviation: Dev Neurobiol
ISSN: 1932-8451
eISSN: 1932-846X
Publisher: United States
Grant note: K08 MH086812 / NIMH NIH HHS
Language: English
Date published: 10/2016
Academic Unit: Psychiatry; Stead Family Department of Pediatrics; Iowa Neuroscience Institute
Record Identifier: 9984003999602771

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