Journal article
Deletion of chromosome arm 17p dna sequences in pediatric high‐grade and juvenile pilocytic astrocytomas
Genes chromosomes & cancer, Vol.12(3), pp.165-172
03/1995
DOI: 10.1002/gcc.2870120303
PMID: 7536455
Abstract
In adults, loss of heterozygosity for DNA on 17p has been shown in high‐grade anaplastic astrocytomas (AAs) and glioblastomas multiforme (GMs), and mutation of the TP53 tumor suppressor gene has been reported in all grades of astrocytomas. Little is known, however, about 17p deletion and TP53 mutation in juvenile pilocytic astrocytomas (JPAs), the most common low‐grade tumors seen in children. To elucidate the genetic characteristics of pediatric high‐grade astrocytomas and JPAs, we performed restriction fragment length polymorphism analysis with probes derived from 17p and TP53 mutational studies in 28 tumor specimens. Telomeric chromosome arm 17p markers 144‐D6 and ABR were lost in 6 (75%) of 8 informative tumors classified as high‐grade (7 AAs, 1 GM) and in 2 (10%) of 20 informative JPAs. Loss of 17p probes centromeric to the TP53 gene were also detected in 3 AAs and 5 JPAs. Four of the 6 (66%) JPAs with losses of 17p DNA sequences recurred rapidly despite aggressive therapy, whereas only 5 of the other 14 (36%) recurred. Mutation of the TP53 gene was detected by polymerase chain reaction and denaturing gradient gel electrophoresis in only 1 JPA and 1 AA. These tumors were also examined for MDM2 gene amplification as an alternate inactivation mechanism for TP53 gene function: no instances of alteration were identified. These results suggest that a gene or genes in addition to TP53 on 17p may be involved in the etiology or progression of high‐grade astrocytomas and aggressive JPAs in children. Further, molecular genetic studies may be an important supplement to current clinical and radiologic data in predicting the outcome and guiding the therapy of children with aggressive and malignant astrocytomas.
Details
- Title: Subtitle
- Deletion of chromosome arm 17p dna sequences in pediatric high‐grade and juvenile pilocytic astrocytomas
- Creators
- Jennifer R WillertLaleh DaneshvarVal C SheffieldPhilip H Cogen
- Resource Type
- Journal article
- Publication Details
- Genes chromosomes & cancer, Vol.12(3), pp.165-172
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; New York
- DOI
- 10.1002/gcc.2870120303
- PMID
- 7536455
- ISSN
- 1045-2257
- eISSN
- 1098-2264
- Number of pages
- 8
- Grant note
- American Cancer Society (PDT‐429) School of Medicine and Cancer Research Coordinating Committee, University of California, San Francisco National Brain Tumor Foundation Clement McCormick Fund
- Language
- English
- Date published
- 03/1995
- Academic Unit
- Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Medical Genetics and Genomics; Ophthalmology and Visual Sciences
- Record Identifier
- 9984065495902771
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