Journal article
Deletion of the PDZ motif of HPV16 E6 preventing immortalization and anchorage-independent growth in human tonsil epithelial cells
Head & neck, Vol.30(2), pp.139-147
02/2008
DOI: 10.1002/hed.20673
PMCID: PMC2600880
PMID: 17657785
Abstract
Background
Human papillomavirus 16 (HPV16) has been associated with head and neck squamous cell carcinoma (HNSCC) in up to 60% of sampled specimens.
Methods
To understand better the viral genes required to transform human tonsil epithelial cells (HTEC), we isolated HTEC's and transduced them with retroviral vectors containing HPV16 E6 and E7.
Results
Immortalization and anchorage‐independent growth of HTEC's only occurred with expression of E6 and E7 with resultant degradation of p53. However, cells expressing E6 lacking the PSD‐95/disc‐large/Zo‐1 (PDZ) motif did not immortalize or grow anchorage independent. Telomerase activity and degradation of p53 were similar for wild‐type and mutant E6.
Conclusion
The mechanism of oncogenic transformation by E6 in HTEC's is dependent on the PDZ binding motif. Identification of pathways affected by the interaction of E6 and PDZ domain containing proteins will further our understanding of how HPV causes HNSCC and will provide potential therapeutic targets.
Details
- Title: Subtitle
- Deletion of the PDZ motif of HPV16 E6 preventing immortalization and anchorage-independent growth in human tonsil epithelial cells
- Creators
- William C Spanos - Department of Microbiology, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IowaJeremy Geiger - Department of Microbiology, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IowaMary E Anderson - Department of Microbiology, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IowaGeorge F Harris - Department of Microbiology, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IowaAaron D Bossler - Department of Microbiology, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IowaRussell B Smith - Department of Microbiology, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IowaAloysius J Klingelhutz - Department of Microbiology, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IowaJohn H Lee - Department of Microbiology, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Head & neck, Vol.30(2), pp.139-147
- DOI
- 10.1002/hed.20673
- PMID
- 17657785
- PMCID
- PMC2600880
- NLM abbreviation
- Head Neck
- ISSN
- 1043-3074
- eISSN
- 1097-0347
- Publisher
- Wiley
- Language
- English
- Date published
- 02/2008
- Academic Unit
- Microbiology and Immunology; Pathology; Radiation Oncology
- Record Identifier
- 9984001152402771
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