Delineation among eight major hematopoietic subsets in murine bone marrow using a two‐color flow cytometric technique

Annette J Schlueter; Sudershan K Bhatia; Xiang Li; Lorraine T Tygrett; Yoshio Yamashita; Peter de Vries; Thomas J Waldschmidt

doi:10.1002/1097-0320(20010401)43:4<297::AID-CYTO1062>3.0.CO;2-9

Journal article

Delineation among eight major hematopoietic subsets in murine bone marrow using a two‐color flow cytometric technique

Annette J Schlueter, Sudershan K Bhatia, Xiang Li, Lorraine T Tygrett, Yoshio Yamashita, Peter de Vries and Thomas J Waldschmidt

Cytometry (New York, N.Y.), Vol.43(4), pp.297-307

04/01/2001

DOI: 10.1002/1097-0320(20010401)43:4<297::AID-CYTO1062>3.0.CO;2-9

PMID: 11260597

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Abstract

Background Many methods have been developed specifically for identifying hematopoietic progenitor cells in murine bone marrow, but few methods allow rapid identification of multiple bone marrow populations. We describe a new, simple method for identifying simultaneously eight populations in murine bone marrow with two‐color flow cytometry and phenotypically define these populations. Methods Bone marrow was stained with anti‐Ly‐6C and anti‐B220 (CD45R) in one fluorochrome and wheat germ agglutinin (WGA) in another fluorochrome. The eight populations identified in this way were defined further primarily by four‐color flow cytometry. Results Six of the eight populations were characterized phenotypically as containing erythroid, granulocytic, mast, early B, mature B, and stem cell populations. Two additional populations with phenotypic characteristics of partially differentiated precursor cells also were identified. One population was Ly‐6C/B220+ and WGA−. It also expressed markers associated with early B, T, and/or dendritic cell differentiation. The second population was Ly‐6ChiWGAhiMac‐1+ and was negative for numerous other lineage‐specific and precursor markers. Its morphology suggested monocytic differentiative potential. Conclusions A two‐color flow cytometric assay profiles six bone marrow populations with identifiable phenotypes and two additional unique, putative hematopoietic precursor populations. Cytometry 43:297–307, 2001. © 2001 Wiley‐Liss, Inc.

lymphopoiesis

hematopoiesis

flow cytometry

dendritic cells

Ly‐6C

bone marrow

Details

Title: Subtitle: Delineation among eight major hematopoietic subsets in murine bone marrow using a two‐color flow cytometric technique
Creators: Annette J Schlueter
Sudershan K Bhatia
Xiang Li
Lorraine T Tygrett
Yoshio Yamashita
Peter de Vries
Thomas J Waldschmidt
Resource Type: Journal article
Publication Details: Cytometry (New York, N.Y.), Vol.43(4), pp.297-307
DOI: 10.1002/1097-0320(20010401)43:4<297::AID-CYTO1062>3.0.CO;2-9
PMID: 11260597
NLM abbreviation: Cytometry
ISSN: 0196-4763
eISSN: 1097-0320
Publisher: John Wiley & Sons, Inc; New York
Number of pages: 11
Grant note: NIH (R01AI31265)
Language: English
Date published: 04/01/2001
Academic Unit: Pathology; Radiation Oncology
Record Identifier: 9984047685002771

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