Journal article
Delta opioid receptors: the link between exercise and cardioprotection
PloS one, Vol.9(11), pp.e113541-e113541
11/21/2014
DOI: 10.1371/journal.pone.0113541
PMCID: PMC4240613
PMID: 25415192
Abstract
This study investigated the role of opioid receptor (OR) subtypes as a mechanism by which endurance exercise promotes cardioprotection against myocardial ischemia-reperfusion (IR) injury. Wistar rats were randomly divided into one of seven experimental groups: 1) control; 2) exercise-trained; 3) exercise-trained plus a non-selective OR antagonist; 4) control sham; 5) exercise-trained plus a kappa OR antagonist; 6) exercise-trained plus a delta OR antagonist; and 7) exercise-trained plus a mu OR antagonist. The exercised animals underwent 4 consecutive days of treadmill training (60 min/day at ∼70% of maximal oxygen consumption). All groups except the sham group were exposed to an in vivo myocardial IR insult, and the myocardial infarct size (IS) was determined histologically. Myocardial capillary density, OR subtype expression, heat shock protein 72 (HSP72) expression, and antioxidant enzyme activity were measured in the hearts of both the exercised and control groups. Exercise training significantly reduced the myocardial IS by approximately 34%. Pharmacological blockade of the kappa or mu OR subtypes did not blunt exercise-induced cardioprotection against IR-mediated infarction, whereas treatment of animals with a non-selective OR antagonist or a delta OR antagonist abolished exercise-induced cardioprotection. Exercise training enhanced the activities of myocardial superoxide dismutase (SOD) and catalase but did not increase the left ventricular capillary density or the mRNA levels of HSP72, SOD, and catalase. In addition, exercise significantly reduced the protein expression of kappa and delta ORs in the heart by 44% and 37%, respectively. Together, these results indicate that ORs contribute to the cardioprotection conferred by endurance exercise, with the delta OR subtype playing a key role in this response.
Details
- Title: Subtitle
- Delta opioid receptors: the link between exercise and cardioprotection
- Creators
- Juliana P Borges - Fundação Oswaldo CruzKarine S Verdoorn - Universidade Federal do Rio de JaneiroAnissa Daliry - Fundação Oswaldo CruzScott K Powers - University of FloridaVictor H Ortenzi - Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de JaneiroRodrigo S Fortunato - Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de JaneiroEduardo Tibiriçá - Fundação Oswaldo CruzMarcos Adriano Lessa - University of Iowa, Anesthesia
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.9(11), pp.e113541-e113541
- DOI
- 10.1371/journal.pone.0113541
- PMID
- 25415192
- PMCID
- PMC4240613
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Language
- English
- Date published
- 11/21/2014
- Academic Unit
- Anesthesia
- Record Identifier
- 9984656533702771
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