Denaturation Temperatures of Dentin Matrices. I. Effect of Demineralization and Dehydration

Steven R Armstrong; Julie L.P Jessop; Erik Winn; Franklin R Tay; David H Pashley

doi:10.1016/j.joen.2005.10.062

Back

Denaturation Temperatures of Dentin Matrices. I. Effect of Demineralization and Dehydration

Journal article

Peer reviewed

Denaturation Temperatures of Dentin Matrices. I. Effect of Demineralization and Dehydration

Steven R Armstrong, Julie L.P Jessop, Erik Winn, Franklin R Tay and David H Pashley

Journal of endodontics, Vol.32(7), pp.638-641

2006

DOI: 10.1016/j.joen.2005.10.062

PMID: 16793470

View Online

Abstract

The denaturation temperature (T d) of dentin collagen in mineralized versus demineralized teeth was examined as a function of dentin age and the extent of dehydration. Using differential scanning calorimetry, T d of mineralized dentin was shown to be between 160°C to 186°C, depending on whether it was from young or old dentin that was hydrated or dehydrated, respectively. Demineralized dentin exhibited a T d of 65.6°C that increased with dehydration to 176°C. The presence of apatite crystallites or interpeptide bonding increased the T d of demineralized matrices. Interpeptide hydrogen bonding seems to stabilize collagen to thermal challenge. Water breaks interpeptide hydrogen bonds making collagen more susceptible to thermal denaturation. Rises in intracanal temperature are unlikely to cause extensive denaturation of mineralized root dentin walls. However, hydrated or partially dehydrated root canal walls that have been partially demineralized with chelating agents or mild acids may be susceptible to thermal denaturation.

dentin

glass transition

Collagen

differential scanning calorimetry

Details

Title: Subtitle: Denaturation Temperatures of Dentin Matrices. I. Effect of Demineralization and Dehydration
Creators: Steven R Armstrong - University of Iowa, Iowa City, Iowa
Julie L.P Jessop - University of Iowa, Iowa City, Iowa
Erik Winn - University of Iowa, Iowa City, Iowa
Franklin R Tay - Department of Oral Biology & Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, Georgia
David H Pashley - Department of Oral Biology & Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, Georgia
Resource Type: Journal article
Publication Details: Journal of endodontics, Vol.32(7), pp.638-641
DOI: 10.1016/j.joen.2005.10.062
PMID: 16793470
NLM abbreviation: J Endod
ISSN: 0099-2399
eISSN: 1878-3554
Publisher: Elsevier Inc
Language: English
Date published: 2006
Academic Unit: Operative Dentistry; Chemical and Biochemical Engineering
Record Identifier: 9984065977902771

Metrics

31 Record Views

31 Times Cited - Web of Science