Journal article
Dendritic arbors of developing retinal ganglion cells are stabilized by β1-integrins
Molecular and cellular neurosciences, Vol.32(3), pp.230-241
07/2006
DOI: 10.1016/j.mcn.2006.04.005
Abstract
The architecture of dendritic arbors is a defining characteristic of neurons and is established through a sequential but overlapping series of events involving process outgrowth and branching, stabilization of the global pattern, and synapse formation. To investigate the roles of cadherins and β1-integrins in maintaining the global architecture of the arbor, we used membrane permeable peptides and transfection with dominant-negative constructs to disrupt adhesion molecule function in intact chick neural retina at a stage when the architecture of the ganglion cell (RGC) arbor is established but synapse formation is just beginning. Inactivation of β1-integrins induces rapid dendrite retraction, with loss of dynamic terminal filopodia followed by resorption of major branches. Disruption of N-cadherin–β-catenin interactions has no effect; however, dendrites do retract following perturbation of the juxtamembrane region of N-cadherin, which disrupts N-cadherin-mediated adhesion and initiates a β1-integrin inactivating signal. Thus, developing RGC dendritic arbors are stabilized by β1-integrin-dependent processes.
Details
- Title: Subtitle
- Dendritic arbors of developing retinal ganglion cells are stabilized by β1-integrins
- Creators
- Glen S Marrs - Department of Biological Sciences, The University of Iowa, Iowa City, IA 52242, USATakashi Honda - Department of Biological Sciences, The University of Iowa, Iowa City, IA 52242, USALeah Fuller - Department of Biological Sciences, The University of Iowa, Iowa City, IA 52242, USARamasamy Thangavel - Department of Biological Sciences, The University of Iowa, Iowa City, IA 52242, USAJanne Balsamo - Department of Biological Sciences, The University of Iowa, Iowa City, IA 52242, USAJack Lilien - Department of Biological Sciences, The University of Iowa, Iowa City, IA 52242, USAMichael E Dailey - Department of Biological Sciences, The University of Iowa, Iowa City, IA 52242, USACarlos Arregui - Instituto de Investigaciones Biotecnológicas, IIB-INTECH, Universidad de San Martín, 1650, San Martín, Buenos Aires, Argentina
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular neurosciences, Vol.32(3), pp.230-241
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.mcn.2006.04.005
- ISSN
- 1044-7431
- eISSN
- 1095-9327
- Language
- English
- Date published
- 07/2006
- Academic Unit
- Biology; Iowa Neuroscience Institute; Neurology
- Record Identifier
- 9984070948602771
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