Dentin Biomodification Potential Depends on Polyphenol Source

T.R Aguiar; C.M.P Vidal; R.S Phansalkar; I Todorova; J.G Napolitano; J.B McAlpine; S.N Chen; G.F Pauli; A.K Bedran-Russo

doi:10.1177/0022034514523783

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Dentin Biomodification Potential Depends on Polyphenol Source

Journal article

Open access

Peer reviewed

Dentin Biomodification Potential Depends on Polyphenol Source

T.R Aguiar, C.M.P Vidal, R.S Phansalkar, I Todorova, J.G Napolitano, J.B McAlpine, S.N Chen, G.F Pauli and A.K Bedran-Russo

Journal of dental research, Vol.93(4), pp.417-422

04/2014

DOI: 10.1177/0022034514523783

PMCID: PMC3957344

PMID: 24574140

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https://europepmc.org/articles/pmc3957344View

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Abstract

Although proanthocyanidins (PACs) modify dentin, the effectiveness of different PAC sources and the correlation with their specific chemical composition are still unknown. This study describes the chemical profiling of natural PAC-rich extracts from 7 plants using ultra high pressure/performance liquid chromatography (UHPLC) to determine the overall composition of these extracts and, in parallel, comprehensively evaluate their effect on dentin properties. The total polyphenol content of the extracts was determined (as gallic acid equivalents) using Folin-Ciocalteau assays. Dentin biomodification was assessed by the modulus of elasticity, mass change, and resistance to enzymatic biodegradation. Extracts with a high polyphenol and PAC content from Vitis vinifera, Theobroma cacao, Camellia sinensis , and Pinus massoniana induced a significant increase in modulus of elasticity and mass. The UHPLC analysis showed the presence of multiple types of polyphenols, ranging from simple phenolic acids to oligomeric PACs and highly condensed tannins. Protective effect against enzymatic degradation was observed for all experimental groups; however, statistically significant differences were observed between plant extracts. The findings provide clear evidence that the dentin bioactivities of PACs are source dependent, resulting from a combination of concentration and specific chemical constitution of the complex PAC mixtures.

Biodegradation

Collagen

Natural Resources

proanthocyanidins

cross-linking reagent

modulus of elasticity

Research Reports

Details

Title: Subtitle: Dentin Biomodification Potential Depends on Polyphenol Source
Creators: T.R Aguiar - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
C.M.P Vidal - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
R.S Phansalkar - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA
I Todorova - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
J.G Napolitano - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA
J.B McAlpine - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA
S.N Chen - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA
G.F Pauli - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA
A.K Bedran-Russo - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA
Resource Type: Journal article
Publication Details: Journal of dental research, Vol.93(4), pp.417-422
Publisher: SAGE Publications; Sage CA: Los Angeles, CA
DOI: 10.1177/0022034514523783
PMID: 24574140
PMCID: PMC3957344
ISSN: 0022-0345
eISSN: 1544-0591
Language: English
Date published: 04/2014
Academic Unit: Operative Dentistry
Record Identifier: 9984065812002771

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