Journal article
Dependence of energy transduction in intact skeletal muscles on the time in tension
Biophysical journal, Vol.18(2), pp.161-172
05/1977
DOI: 10.1016/S0006-3495(77)85605-1
PMCID: PMC1473282
PMID: 140712
Abstract
In intact single crayfish muscle fibers and frog semitendinosus muscles we have studied the tension response to sinusoidal length changes in the frequency range of 0.25-133 Hz. By this method we have resolved three processes in the interaction of myosin cross-bridges with actin in fully activated preparations. They are (A) a low-frequency phase advance, (B) a middle-frequency delay, and (C) a high-frequency advance. These processes can be used as probes to study the chemomechanical coupling of contractility. Process (B) represents net power output from the muscle preparation (oscillatory work). With maximal K or caffeine activation of crayfish muscle at 20 degrees C, it decreases to zero in the initial 45 s of maintained tension. Similar results were obtained with frog semitendinosus whole muscles. We interpret this decrease of (B) with time as a gradual decrease in actomyosin ATP-hydrolysis rate.
Details
- Title: Subtitle
- Dependence of energy transduction in intact skeletal muscles on the time in tension
- Creators
- Masataka KawaiPhilip W BrandtMorton Orentlicher
- Resource Type
- Journal article
- Publication Details
- Biophysical journal, Vol.18(2), pp.161-172
- Publisher
- United States
- DOI
- 10.1016/S0006-3495(77)85605-1
- PMID
- 140712
- PMCID
- PMC1473282
- ISSN
- 0006-3495
- eISSN
- 1542-0086
- Language
- English
- Date published
- 05/1977
- Academic Unit
- Anatomy and Cell Biology; Internal Medicine
- Record Identifier
- 9984025691902771
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