Depletion of Alveolar Glycogen Corresponds With Immunohistochemical Development of CD208 Antigen Expression in Perinatal Lamb Lung

David K Meyerholz; James A DeGraaf; Jack M Gallup; Alicia K Olivier; Mark R Ackermann

doi:10.1369/jhc.6A7002.2006

Back

Depletion of Alveolar Glycogen Corresponds With Immunohistochemical Development of CD208 Antigen Expression in Perinatal Lamb Lung

Journal article

Open access

Peer reviewed

Depletion of Alveolar Glycogen Corresponds With Immunohistochemical Development of CD208 Antigen Expression in Perinatal Lamb Lung

David K Meyerholz, James A DeGraaf, Jack M Gallup, Alicia K Olivier and Mark R Ackermann

The journal of histochemistry and cytochemistry, Vol.54(11), pp.1247-1253

11/2006

DOI: 10.1369/jhc.6A7002.2006

PMCID: PMC2791090

PMID: 16899762

Files and links (1)

url

https://doi.org/10.1369/jhc.6A7002.2006View

Published (Version of record) Open Access

Abstract

CD208 DC lysosomal-associated protein is a marker of activated human dendritic cells; however, recently it was described as a marker of adult type II pneumocytes in many species including humans and sheep. Our hypothesis was that CD208 is developmentally regulated in lung pneumocytes. Lamb lungs at varying stages of development were stained immunohistochemically for CD208 and with Nile red (a fluorescent stain for lamellar bodies of type II cells) along with pulmonary markers of maturation (glycogen stores and surfactant protein A [SP-A] expression) or proliferation (Ki-67). CD208 staining and Nile red were localized to rare pneumocytes in young fetal lambs (day 115), increasing in frequency and stain intensity with age. Periodic acid-Schiff staining of glycogen granules was most prominent in the young lambs (day 115) with reduced staining through advancing lung development. SP-A was detected in pulmonary epithelia and staining in alveoli increased through gestation with decreased staining at 2 weeks of age. Intranuclear Ki-67 staining decreased through late gestation but was increased in 2-week-old lambs. Ontogeny of CD208 staining and depletion of glycogen were correlated (p<0.0001) and consistent with the premise that CD208 is localized to developing lamellar bodies. The findings suggest that CD208 antigen expression may serve as a marker for pneumocyte maturation in the developing fetal lung.

Details

Title: Subtitle: Depletion of Alveolar Glycogen Corresponds With Immunohistochemical Development of CD208 Antigen Expression in Perinatal Lamb Lung
Creators: David K Meyerholz - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, Iowa
James A DeGraaf - Department of Veterinary Pathology, Iowa State University, Ames, Iowa
Jack M Gallup - Department of Veterinary Pathology, Iowa State University, Ames, Iowa
Alicia K Olivier - Department of Veterinary Pathology, Iowa State University, Ames, Iowa
Mark R Ackermann - Department of Veterinary Pathology, Iowa State University, Ames, Iowa
Resource Type: Journal article
Publication Details: The journal of histochemistry and cytochemistry, Vol.54(11), pp.1247-1253
DOI: 10.1369/jhc.6A7002.2006
PMID: 16899762
PMCID: PMC2791090
NLM abbreviation: J Histochem Cytochem
ISSN: 0022-1554
eISSN: 1551-5044
Language: English
Date published: 11/2006
Academic Unit: Pathology
Record Identifier: 9984083812102771

Metrics

30 Record Views

16 Times Cited - Web of Science