Depletion of alveolar macrophages ameliorates virus-induced disease following a pulmonary coronavirus infection

Stacey M Hartwig; Kaitlyn M Holman; Steven M Varga

doi:10.1371/journal.pone.0090720

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Depletion of alveolar macrophages ameliorates virus-induced disease following a pulmonary coronavirus infection

Journal article

Open access

Peer reviewed

Depletion of alveolar macrophages ameliorates virus-induced disease following a pulmonary coronavirus infection

Stacey M Hartwig, Kaitlyn M Holman and Steven M Varga

PloS one, Vol.9(3), pp.e90720-e90720

2014

DOI: 10.1371/journal.pone.0090720

PMCID: PMC3946553

PMID: 24608125

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Abstract

Coronaviruses cause respiratory disease in humans that can range from mild to severe. However, the pathogenesis of pulmonary coronavirus infections is poorly understood. Mouse hepatitis virus type 1 (MHV-1) is a group 2 coronavirus capable of causing severe morbidity and mortality in highly susceptible C3H/HeJ mice. We have previously shown that both CD4 and CD8 T cells play a critical role in mediating MHV-1-induced disease. Here we evaluated the role of alveolar macrophages (AM) in modulating the adaptive immune response and subsequent disease. Depletion of AM using clodronate liposomes administered prior to MHV-1 infection was associated with a significant amelioration of MHV-1-induced morbidity and mortality. AM depletion resulted in a decreased number of virus-specific CD4 T cells in the lung airways. In addition, a significant increase in the frequency and total number of Tregs in the lung tissue and lung airways was observed following MHV-1 infection in mice depleted of AM. Our results indicate that AM play a critical role in modulating MHV-1-induced morbidity and mortality.

CD4-Positive T-Lymphocytes - metabolism

Cells, Cultured

Murine hepatitis virus - pathogenicity

Coronavirus Infections - immunology

Mice, Inbred C3H

Lung - virology

Animals

Coronavirus

CD8-Positive T-Lymphocytes - metabolism

Female

Mice

Lung - immunology

Macrophages, Alveolar - immunology

Details

Title: Subtitle: Depletion of alveolar macrophages ameliorates virus-induced disease following a pulmonary coronavirus infection
Creators: Stacey M Hartwig - Department of Microbiology, University of Iowa, Iowa City, Iowa, United States of America
Kaitlyn M Holman - Department of Microbiology, University of Iowa, Iowa City, Iowa, United States of America
Steven M Varga - Department of Microbiology, University of Iowa, Iowa City, Iowa, United States of America; Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, Iowa, United States of America; Department of Pathology, University of Iowa, Iowa City, Iowa, United States of America
Resource Type: Journal article
Publication Details: PloS one, Vol.9(3), pp.e90720-e90720
DOI: 10.1371/journal.pone.0090720
PMID: 24608125
PMCID: PMC3946553
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science
Grant note: R21 AI083482 / NIAID NIH HHS R56 AI106776 / NIAID NIH HHS
Language: English
Date published: 2014
Academic Unit: Graduate College Admin and Gen; Microbiology and Immunology; Pathology
Record Identifier: 9984083875602771

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