Depletion of blood-borne macrophages does not reduce demyelination in mice infected with a neurotropic coronavirus

Shurong Xue; Ning Sun; Nico Van Rooijen; Stanley Perlman

doi:10.1128/JVI.73.8.6327-6334.1999

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Depletion of blood-borne macrophages does not reduce demyelination in mice infected with a neurotropic coronavirus

Journal article

Open access

Peer reviewed

Depletion of blood-borne macrophages does not reduce demyelination in mice infected with a neurotropic coronavirus

Shurong Xue, Ning Sun, Nico Van Rooijen and Stanley Perlman

Journal of virology, Vol.73(8), pp.6327-6334

08/1999

DOI: 10.1128/JVI.73.8.6327-6334.1999

PMCID: PMC112711

PMID: 10400724

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Abstract

Mice infected with the neurotropic coronavirus mouse hepatitis virus strain JHM (MHV-JHM) develop a chronic demyelinating disease with symptoms of hindlimb paralysis. Histological examination of the brains and spinal cords of these animals reveals the presence of large numbers of activated macrophages/microglia. In two other experimental models of demyelination, experimental allergic encephalomyelitis and Theiler's murine encephalomyelitis virus-induced demyelination, depletion of hematogenous macrophages abrogates the demyelinating process. In both of these diseases, early events in the demyelinating process are inhibited by macrophage depletion. From these studies, it was not possible to determine whether infiltrating macrophages were required for late steps in the process, such as myelin removal. In this study, we show that when macrophages are depleted with either unmodified or mannosylated liposomes encapsulating dichloromethylene diphosphate, the amount of demyelination detected in MHV-infected mice is not affected. At a time when these cells were completely depleted from the liver, approximately equivalent numbers of macrophages were present in the spinal cords of control and drug-treated animals. These results suggest that blood-borne macrophages are not required for MHV-induced demyelination and also suggest that other cells, such as perivascular macrophages or microglia, perform the function of these cells in the presence of drug.

Clodronic Acid

Coronavirus - pathogenicity

Histocompatibility Antigens Class I - immunology

Central Nervous System - pathology

Murine hepatitis virus - pathogenicity

Coronavirus Infections - immunology

Coronavirus - immunology

Murine hepatitis virus - immunology

Animals

Histocompatibility Antigens Class II - immunology

Microglia - immunology

Time Factors

Demyelinating Diseases - immunology

Mice

Mice, Inbred BALB C

Chronic Disease

Macrophages - immunology

Details

Title: Subtitle: Depletion of blood-borne macrophages does not reduce demyelination in mice infected with a neurotropic coronavirus
Creators: Shurong Xue - Departments of Microbiology, University of Iowa, Iowa City, Iowa 52242, USA
Ning Sun
Nico Van Rooijen
Stanley Perlman
Resource Type: Journal article
Publication Details: Journal of virology, Vol.73(8), pp.6327-6334
DOI: 10.1128/JVI.73.8.6327-6334.1999
PMID: 10400724
PMCID: PMC112711
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: United States
Grant note: NS 36592 / NINDS NIH HHS R01 NS036592 / NINDS NIH HHS
Language: English
Date published: 08/1999
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777354802771

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