Depletion of neutrophils in IL-10(-/-) mice delays clearance of gastric Helicobacter infection and decreases the Th1 immune response to Helicobacter

Hanan F Ismail; Pamela Fick; Juan Zhang; Richard G Lynch; Daniel J Berg

doi:10.4049/jimmunol.170.7.3782

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Depletion of neutrophils in IL-10(-/-) mice delays clearance of gastric Helicobacter infection and decreases the Th1 immune response to Helicobacter

Journal article

Peer reviewed

Depletion of neutrophils in IL-10(-/-) mice delays clearance of gastric Helicobacter infection and decreases the Th1 immune response to Helicobacter

Hanan F Ismail, Pamela Fick, Juan Zhang, Richard G Lynch and Daniel J Berg

The Journal of immunology (1950), Vol.170(7), pp.3782-3789

04/01/2003

DOI: 10.4049/jimmunol.170.7.3782

PMID: 12646644

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Abstract

Gastric infection with Helicobacter induces a lymphocyte-rich mucosal inflammation that contains a minor population of neutrophilic granulocytes. The function of neutrophils in the local immune response to gastric Helicobacter infection remains unknown. To investigate this issue, we conducted experiments in neutrophil-depleted control wild-type (wt) and IL-10(-/-) mice infected with Helicobacter felis by gastric lavage. Infection of wt mice elicited a mild, focal gastritis and a Helicobacter-specific Th1 immune response. In wt mice Helicobacter colonization of the stomach was persistent and progressively increased during the 29 days of observation. Infection of IL-10(-/-) mice with H. felis elicited a severe chronic gastritis and a greatly enhanced Helicobacter-specific Th1 immune response, as compared with wt mice. After initial colonization, the IL-0(-/-) mice completely cleared Helicobacter from the stomach by day 8. The gastric inflammation in wt and IL-10(-/-) mice contained modest numbers of neutrophils. The intensity of gastric inflammation and the extent of Helicobacter colonization were similar in control and in neutrophil-depleted wt mice. In contrast, neutrophil depletion of Helicobacter-infected IL-10(-/-) mice decreased the severity of gastritis, modulated the Helicobacter-specific Th1 immune response, and delayed the clearance of bacteria from the stomach. These studies identify a role for neutrophils in the local and systemic immune response to gastric Helicobacter in IL-10(-/-) mice.

Antigens, Bacterial - immunology

Gastritis - genetics

Helicobacter Infections - prevention & control

Helicobacter - immunology

Lymphocyte Depletion

Th1 Cells - immunology

Gastritis - prevention & control

Th1 Cells - metabolism

Helicobacter - growth & development

Gastritis - microbiology

Leukocytosis - microbiology

Neutropenia - genetics

Neutropenia - immunology

Neutrophils - pathology

Interleukin-10 - deficiency

Cells, Cultured

Leukocytosis - immunology

Helicobacter Infections - immunology

Helicobacter Infections - genetics

Down-Regulation - genetics

Mice, Knockout

Th1 Cells - microbiology

Animals

T-Lymphocyte Subsets - pathology

Down-Regulation - immunology

Gastritis - immunology

Interleukin-10 - genetics

Mice

T-Lymphocyte Subsets - microbiology

Leukocytosis - genetics

Helicobacter Infections - microbiology

Details

Title: Subtitle: Depletion of neutrophils in IL-10(-/-) mice delays clearance of gastric Helicobacter infection and decreases the Th1 immune response to Helicobacter
Creators: Hanan F Ismail - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA
Pamela Fick
Juan Zhang
Richard G Lynch
Daniel J Berg
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.170(7), pp.3782-3789
DOI: 10.4049/jimmunol.170.7.3782
PMID: 12646644
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: R29 CA76129 / NCI NIH HHS
Language: English
Date published: 04/01/2003
Academic Unit: Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
Record Identifier: 9984094571202771

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