Journal article
Derivation of induced pluripotent stem cells from ferret somatic cells
American journal of physiology. Lung cellular and molecular physiology, Vol.318(4), pp.L671-L683
04/01/2020
DOI: 10.1152/AJPLUNG.00456.2019
PMCID: PMC7191474
PMID: 32073882
Abstract
Ferrets are an attractive mammalian model for several diseases, especially those affecting the lungs, liver, brain, and kidneys. Many chronic human diseases have been difficult to model in rodents due to differences in size and cellular anatomy. This is particularly the case for the lung, where ferrets provide an attractive mammalian model of both acute and chronic lung diseases, such as influenza, cystic fibrosis, A1A emphysema, and obliterative bronchiolitis, closely recapitulating disease pathogenesis, as it occurs in humans. As such, ferrets have the potential to be a valuable preclinical model for the evaluation of cell-based therapies for lung regeneration and, likely, for other tissues. Induced pluripotent stem cells (iPSCs) provide a great option for provision of enough autologous cells to make patient-specific cell therapies a reality. Unfortunately, they have not been successfully created from ferrets. In this study, we demonstrate the generation of ferret iPSCs that reflect the primed pluripotent state of human iPSCs. Ferret fetal fibroblasts were reprogrammed and acquired core features of pluripotency, having the capacity for self-renewal, multilineage differentiation, and a high-level expression of the core pluripotency genes and pathways at both the transcriptional and protein level. In conclusion, we have generated ferret pluripotent stem cells that provide an opportunity for advancing our capacity to evaluate autologous cell engraftment in ferrets.
Details
- Title: Subtitle
- Derivation of induced pluripotent stem cells from ferret somatic cells
- Creators
- Jinghui Gao - Hastings CenterSophia Petraki - Hastings CenterXingshen Sun - University of IowaLeonard A Brooks - University of IowaThomas J Lynch - University of IowaChih-Lin Hsieh - University of Southern CaliforniaReem Elteriefi - Hastings CenterZareeb Lorenzana - Hastings CenterVasu Punj - Hastings CenterJohn F Engelhardt - University of IowaKalpaj R Parekh - University of IowaAmy L Ryan - Hastings Center
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Lung cellular and molecular physiology, Vol.318(4), pp.L671-L683
- DOI
- 10.1152/AJPLUNG.00456.2019
- PMID
- 32073882
- PMCID
- PMC7191474
- ISSN
- 1040-0605
- eISSN
- 1522-1504
- Grant note
- P30 ES005605 / NIEHS NIH HHS R24 HL123482 / NHLBI NIH HHS P30 DK054759 / NIDDK NIH HHS R01 HL136370 / NHLBI NIH HHS
- Language
- English
- Date published
- 04/01/2020
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Cardiothoracic Surgery; Internal Medicine
- Record Identifier
- 9984284451402771
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