Journal article
Derivatization and purification of bisecting tyrosinamide-oligosaccharides from ovotransferrin
Archives of biochemistry and biophysics, Vol.315(2), pp.460-466
12/1994
DOI: 10.1006/abbi.1994.1525
PMID: 7986092
Abstract
The major N-linked oligosaccharides from ovotransferrin were purified on a large scale. The oligosaccharides were released from 5 g of the glycoprotein using N-glycosidase F and isolated from a mixed bed ion exchange column. Reducing-oligosaccharides were reacted with ammonium bicarbonate to form a reducing-end glycosylamine which coupled with the N-hydroxysuccinimidyl ester of Boc-tyrosine, resulting in tyrosinamide-oligosaccharides. Resolution of tyrosinamide-oligosaccharides on reverse-phase (RP)-HPLC necessitated the removal of Boc by treatment with trifloroacetic acid. Two major tyrosinamide-oligosaccharides were isolated from preparative RP-HPLC and were characterized by 500 MHz proton NMR and FAB-MS. These were a biantennary and triantennary oligosaccharide, each possessing a bisecting GlcNAc residue extending from the central core Man residue. Thus, ovotransferrin represents an abundant glycoprotein source from which to prepare multi-micromole quantities of bisecting complex oligosaccharides which may find utility in exploring the ligand specificity of mammalian lectins or may be used as synthons to prepare novel glycoconjugates.
Details
- Title: Subtitle
- Derivatization and purification of bisecting tyrosinamide-oligosaccharides from ovotransferrin
- Creators
- M L Corradi da Silva - Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, Ohio State University, Columbus 43210T TamuraK G Rice
- Resource Type
- Journal article
- Publication Details
- Archives of biochemistry and biophysics, Vol.315(2), pp.460-466
- DOI
- 10.1006/abbi.1994.1525
- PMID
- 7986092
- NLM abbreviation
- Arch Biochem Biophys
- ISSN
- 0003-9861
- eISSN
- 1096-0384
- Publisher
- United States
- Grant note
- GM48048 / NIGMS NIH HHS
- Language
- English
- Date published
- 12/1994
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984065309402771
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