Journal article
Desiccation tolerance is associated with Staphylococcus aureus hypertransmissibility, resistance and infection development in the operating room
Journal of Hospital Infection, Vol.100(3), pp.299-308
11/2018
DOI: 10.1016/j.jhin.2018.06.020
PMID: 29966756
Abstract
Desiccation tolerance increases Staphylococcus aureus survival and risk of transmission. A better understanding of factors driving intraoperative transmission of S. aureus pathogens may lead to innovative improvements in intraoperative infection control. To determine whether desiccation tolerance is associated with intraoperative S. aureus transmission, and to examine typical transmission dynamics for desiccation-tolerant isolates in the operating room in order to provide the impetus for development of improved intraoperative infection control strategies. S. aureus isolates (N=173) were collected from anaesthesia work area reservoirs in 274 operating room environments. Desiccation tolerance was assessed and the potential association with sequence type (ST) and clonal transmission was evaluated. Whole cell genome analysis and pulsed-field gel electrophoresis analysis were used to compare desiccation-tolerant isolates with causative organisms of infection. S. aureus ST 5 isolates had greater desiccation tolerance than all other intraoperative STs [ST 5, N=34, median Day 2 colony-forming unit (cfu) survival 0.027% ± 0.029%; other STs, N=139, median Day 2 cfu survival 0.0091% ± 1.41%; corrected P=0.0001]. ST 5 was associated with increased risk of clonal transmission (relative risk 1.82, 95% confidence interval 1.23–2.71, P=0.003). ST 5 transmission was linked by whole cell genome analysis to postoperative infection. Increased desiccation tolerance is associated with intraoperative transmission of S. aureus ST 5 isolates that are linked to postoperative infection. Future work should determine whether attenuation of desiccation-tolerant, intraoperative ST 5 strains can impact the incidence of healthcare-associated infections.
Details
- Title: Subtitle
- Desiccation tolerance is associated with Staphylococcus aureus hypertransmissibility, resistance and infection development in the operating room
- Creators
- R.W Loftus - Department of Anesthesia, University of Iowa, Iowa City, IA, USAF Dexter - Department of Anesthesia, University of Iowa, Iowa City, IA, USAA.D.M Robinson - Department of Anesthesia, University of Iowa, Iowa City, IA, USAA.R Horswill - Department of Immunology and Microbiology, University of Colorado School of Medicine and the VA Eastern Colorado Healthcare System, Denver, CO, USA
- Resource Type
- Journal article
- Publication Details
- Journal of Hospital Infection, Vol.100(3), pp.299-308
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/j.jhin.2018.06.020
- PMID
- 29966756
- ISSN
- 0195-6701
- eISSN
- 1532-2939
- Grant note
- DOI: 10.13039/100008893, name: University of Iowa
- Language
- English
- Date published
- 11/2018
- Academic Unit
- Health Management and Policy; Anesthesia
- Record Identifier
- 9983806247902771
Metrics
25 Record Views