Journal article
Design and validation of a bioreactor for engineering vocal fold tissues under combined tensile and vibrational stresses
Journal of biomechanics, Vol.37(10), pp.1521-1529
10/01/2004
DOI: 10.1016/j.jbiomech.2004.01.007
PMID: 15336927
Abstract
Criteria are outlined for the design of a bioreactor that can simulate the vibrational stresses in vocal fold movement during speech. Requirements are 0–1
mm amplitudes in the 20–200
Hz frequency range, a variable on–off stress regime, and maintenance of tissue viability over several days. The bioreactor uses dual drivers, one for low frequency (or static) strains, and another for high-frequencies vibrational strains. Response is linear at the driving end for an input of 0–5
V. The amplitude decreases linearly with frequency at constant input voltage, and the phase changes by nearly 180° over the 20–200
Hz range. Human vocal fold fibroblasts were cultured in a polymer substrate and subjected to static and vibrational forces. The results indicate that vibratory strain alters the expression levels of many extracellular matrix-related genes, as well as the spatial distribution of cells and matrix.
Details
- Title: Subtitle
- Design and validation of a bioreactor for engineering vocal fold tissues under combined tensile and vibrational stresses
- Creators
- Ingo R. Titze - University of IowaRobert W. Hitchcock - University of UtahKelly Broadhead - University of UtahKen Webb - University of UtahWenhua Li - University of UtahSteven D. Gray - University of UtahPatrick A. Tresco - University of Utah
- Resource Type
- Journal article
- Publication Details
- Journal of biomechanics, Vol.37(10), pp.1521-1529
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/j.jbiomech.2004.01.007
- PMID
- 15336927
- ISSN
- 0021-9290
- eISSN
- 1873-2380
- Number of pages
- 9
- Language
- English
- Date published
- 10/01/2004
- Academic Unit
- School of Music; Communication Sciences and Disorders
- Record Identifier
- 9984719565602771
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