Detection and analysis of antigen-specific CD8+ T cells

Vladimir Badovinac; John Harty

doi:10.1385/IR:24:3:325

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Detection and analysis of antigen-specific CD8+ T cells

Journal article

Peer reviewed

Detection and analysis of antigen-specific CD8+ T cells

Vladimir Badovinac and John Harty

Immunologic research, Vol.24(3), pp.325-332

12/2001

DOI: 10.1385/IR:24:3:325

PMID: 11817329

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Abstract

Based on recent advances in techniques that can detect and enumerate antigen-specific CD8+ T cells, it is evident that these cells can differentially regulated CD8+ T cell effector mechanisms at the single-cell level. Interplay between effector mechanisms that are employed by antigen-specific CD8+T cells during the immune response in vivo can be addressed with different techniques that “count” cells either directly (T cell receptor (TCR) expression) or indirectly (antigen-specific cytokine production).

Ag-specific CD8 + T cells

IFNγ

ON/OFF cycling

Internal Medicine

Intracellular cytokine staining

Medicine/Public Health, general

TNF

Allergology

Infection

Immunology

Medicine & Public Health

MHC class I tetramers

Perforin

Details

Title: Subtitle: Detection and analysis of antigen-specific CD8+ T cells
Creators: Vladimir Badovinac - Department of Interdisciplinary Graduate Program in Immunology University of Iowa Iowa City IA
John Harty - Department of Interdisciplinary Graduate Program in Immunology University of Iowa Iowa City IA
Resource Type: Journal article
Publication Details: Immunologic research, Vol.24(3), pp.325-332
Publisher: Humana Press; Totowa
DOI: 10.1385/IR:24:3:325
PMID: 11817329
ISSN: 0257-277X
eISSN: 1559-0755
Language: English
Date published: 12/2001
Academic Unit: Microbiology and Immunology; Pathology
Record Identifier: 9984047778702771

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