Journal article
Detection of 3,3'-Dichlorobiphenyl in Human Maternal Plasma and Its Effects on Axonal and Dendritic Growth in Primary Rat Neurons
Toxicological sciences, Vol.158(2), pp.401-411
08/2017
DOI: 10.1093/toxsci/kfx100
PMCID: PMC5837364
PMID: 28510766
Abstract
3,3'-Dichlorobiphenyl (PCB 11), a byproduct of pigment production, is increasingly detected in environmental samples. While more highly chlorinated PCB congeners are known developmental neurotoxicants, nothing is known about the potential developmental neurotoxicity of PCB 11. To address this critical data gap, we measured PCB 11 levels in human maternal plasma and quantified the effects of PCB 11 and its major metabolites on morphometric parameters of neuronal connectivity in cultured primary neurons. Mass spectrometry analyses of plasma from 241 pregnant women enrolled in the MARBLES study (University of California, Davis) detected PCB 11 in all samples at concentrations ranging from 0.005 to 1.717 ng/ml. Morphometric analyses of primary neuron-glia co-cultures dissociated from the neocortices or hippocampi of neonatal Sprague Dawley rats exposed to vehicle or concentrations ranging from 1 attamolar (aM) to 1 micromolar (µM) of PCB 11, OH-PCB 11, or PCB 11 sulfate indicated that PCB 11 and both metabolites significantly increased axonal and dendritic growth in cortical and hippocampal pyramidal neurons. PCB 11 significantly altered neuronal morphogenesis at concentrations as low as 1 femtomolar (fM), which is ∼0.22 ng/ml. These data suggest the potential for the developing human brain to be exposed to PCB 11, and demonstrate that environmentally relevant levels of PCB 11 alter axonal and dendritic growth in neuronal cell types critically involved in cognitive and higher-order behaviors. These findings identify PCB 11 as a potential environmental risk factor for adverse neurodevelopmental outcomes in humans.
Details
- Title: Subtitle
- Detection of 3,3'-Dichlorobiphenyl in Human Maternal Plasma and Its Effects on Axonal and Dendritic Growth in Primary Rat Neurons
- Creators
- Sunjay Sethi - Department of Occupational & Environmental Health, University of Iowa, Iowa City, Iowa 52242Kimberly P Keil - Department of Occupational & Environmental Health, University of Iowa, Iowa City, Iowa 52242Hao Chen - Department of Occupational & Environmental Health, University of Iowa, Iowa City, Iowa 52242Keri Hayakawa - Department of Occupational & Environmental Health, University of Iowa, Iowa City, Iowa 52242Xueshu Li - Department of Occupational & Environmental Health, University of Iowa, Iowa City, Iowa 52242Yanping Lin - Department of Occupational & Environmental Health, University of Iowa, Iowa City, Iowa 52242Hans-Joachim Lehmler - Department of Occupational & Environmental Health, University of Iowa, Iowa City, Iowa 52242Birgit Puschner - Department of Occupational & Environmental Health, University of Iowa, Iowa City, Iowa 52242Pamela J Lein - Department of Occupational & Environmental Health, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Toxicological sciences, Vol.158(2), pp.401-411
- DOI
- 10.1093/toxsci/kfx100
- PMID
- 28510766
- PMCID
- PMC5837364
- NLM abbreviation
- Toxicol Sci
- ISSN
- 1096-6080
- eISSN
- 1096-0929
- Publisher
- Oxford University Press
- Grant note
- RD 83543201 / ; ; ; U54 HD079125; F32 HD088016 / ; ; ;
- Language
- English
- Date published
- 08/2017
- Academic Unit
- Occupational and Environmental Health; Iowa Neuroscience Institute; Iowa Superfund Research Program
- Record Identifier
- 9984000923402771
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