Journal article
Detection of JC Virus DNA and Proteins in the Bone Marrow of HIV-Positive and HIV-Negative Patients: Implications for Viral Latency and Neurotropic Transformation
The Journal of infectious diseases, Vol.199(6), pp.881-888
03/15/2009
DOI: 10.1086/597117
PMCID: PMC2893283
PMID: 19434914
Abstract
Background. We sought to determine the prevalence of JC virus (JCV) in bone marrow samples from human immunodeficiency virus (HIV)-positive and HIV-negative patients and to determine whether bone marrow is a site of latency and neurotropic transformation of JCV, the agent of progressive multifocal leukoencephalopathy (PML).
Methods. We collected bone marrow aspirates, archival bone marrow samples, and blood and urine samples from 75 HIV-negative and 47 HIV-positive patients without PML as well as bone marrow and urine or kidney samples from 8 HIV-negative and 15 HIV-positive patients with PML. Samples were tested for JCV DNA by quantitative polymerase chain reaction and for JCV protein expression by immunohistochemical analysis. JCV regulatory regions (RRs) were characterized by sequencing.
Results. JCV DNA was detected in bone marrow samples from 10 (13%) of 75 and 22 (47%) of 47 of the HIV-negative and HIV-positive patients without PML, respectively, compared with 3 (38%) of 8 and 4 (27%) of 15 of the HIV-negative and HIV-positive patients with PML. JCV DNA (range, 2-1081 copies/mu g of cellular DNA) was detected in multiple leukocyte subpopulations of blood and bone marrow samples. JCV large T antigen, but not VP1 capsid protein, was expressed in bone marrow plasma cells. Bone marrow JCV RR sequences were similar to those usually found in the brains of patients with PML.
Conclusions. Bone marrow is an important reservoir and a possible site of neurotropic transformation for JCV.
Details
- Title: Subtitle
- Detection of JC Virus DNA and Proteins in the Bone Marrow of HIV-Positive and HIV-Negative Patients: Implications for Viral Latency and Neurotropic Transformation
- Creators
- Chen S. Tan - Beth Israel Deaconess Medical CenterBruce J. Dezube - Beth Israel Deaconess Medical CenterParul Bhargava - Beth Israel Deaconess Medical CenterPatrick Autissier - Beth Israel Deaconess Medical CenterChristian Wuethrich - Beth Israel Deaconess Medical CenterJanice Miller - Beth Israel Deaconess Medical CenterIgor J. Koralnik - Beth Israel Deaconess Medical Center
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.199(6), pp.881-888
- Publisher
- Oxford Univ Press
- DOI
- 10.1086/597117
- PMID
- 19434914
- PMCID
- PMC2893283
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Number of pages
- 8
- Grant note
- R01 NS041198; K24 NS060950-01; K24 NS060950; R24 NS038841; R24NS38841; K24 NS 060950; R01 NS047029-04; R01 NS047029; R01 NS041198-08 / NINDS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) T32 AI007061; P30 AI60354; T32 AI07091-30; P30 AI060354 / NIAID NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) P30AI060354 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) R01NS041198 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) U01MH083545 / NATIONAL INSTITUTE OF MENTAL HEALTH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH) R24 MH059724; R24MH59745; R24 MH059745; U01 MH083545; R24MH59656; R24MH59724 / NIMH NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH) 047029 / PHS HHS; United States Department of Health & Human Services; United States Public Health Service
- Language
- English
- Date published
- 03/15/2009
- Academic Unit
- Iowa Neuroscience Institute; Internal Medicine
- Record Identifier
- 9984366284902771
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