Journal article
Detection of Neonatal Drug Exposure Using Umbilical Cord Tissue and Liquid Chromatography Time-of-Flight Mass Spectrometry
Therapeutic drug monitoring, Vol.36(1), pp.119-124
2014
DOI: 10.1097/FTD.0b013e3182a0d18c
PMID: 24061447
Abstract
Background:
A method for qualitative detection of 57 drugs and metabolites in umbilical cord tissue using liquid chromatography time-of-flight (TOF) mass spectrometry is described.
Methods:
Results from 32 deidentified positive specimens analyzed by an outside laboratory using “screen with reflex to confirmation” testing were compared with TOF results. In addition, 57 umbilical cord tissue specimens paired with corresponding chart review data and 37 with meconium test results were analyzed by TOF. Urine drug test results from mother (n = 18) and neonate (n = 30) were included if available. Cutoff concentrations, recovery, and matrix effects were determined by analyzing fortified drug-free cord tissue and negative specimens. Cutoffs (in nanograms per gram) ranged from 1 to 10 for opioids and opioid antagonists, 5–10 for benzodiazepines and nonbenzodiazepine hypnotics, 20–40 for barbiturates, 8 for stimulants, and 4 for phencyclidine. Adequate sensitivity for the detection of cannabis exposure could not be realized with this method.
Conclusions:
Liquid chromatography time-of-flight mass spectrometry can provide accurate and sensitive detection of in utero drug exposure using umbilical cord tissue.
Details
- Title: Subtitle
- Detection of Neonatal Drug Exposure Using Umbilical Cord Tissue and Liquid Chromatography Time-of-Flight Mass Spectrometry
- Creators
- Stephanie J MARIN - ARUP Institute for Clinical and Experimental Pathology, Inc, Salt Lake City, Utah, United StatesAnna METCALF - AR-UP Laboratories, Inc, Salt Lake City, Utah, United StatesMatthew D KRASOWSKI - Department of Pathology, University of Iowa Hospitals and Clinics, Iowa, Iowa, United StatesBrian S LINERT - Department of Pathology, University of Iowa Hospitals and Clinics, Iowa, Iowa, United StatesChantry J CLARK - AR-UP Laboratories, Inc, Salt Lake City, Utah, United StatesFrederick G STRATHMANN - ARUP Institute for Clinical and Experimental Pathology, Inc, Salt Lake City, Utah, United StatesGwendolyn A MCMILLIN - ARUP Institute for Clinical and Experimental Pathology, Inc, Salt Lake City, Utah, United States
- Resource Type
- Journal article
- Publication Details
- Therapeutic drug monitoring, Vol.36(1), pp.119-124
- Publisher
- Lippincott Williams & Wilkins; Hagerstown, MD
- DOI
- 10.1097/FTD.0b013e3182a0d18c
- PMID
- 24061447
- ISSN
- 0163-4356
- eISSN
- 1536-3694
- Language
- English
- Date published
- 2014
- Academic Unit
- Pathology
- Record Identifier
- 9984047741802771
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