Detection of SPM-1-producing Pseudomonas aeruginosa and class D β-lactamase-producing Acinetobacter baumannii isolates by use of liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization-time of flight mass spectrometry

Cecilia G Carvalhaes; Rodrigo Cayô; Diego M Assis; Evelin R Martins; Luiz Juliano; Maria A Juliano; Ana C Gales

doi:10.1128/JCM.02365-12

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Detection of SPM-1-producing Pseudomonas aeruginosa and class D β-lactamase-producing Acinetobacter baumannii isolates by use of liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization-time of flight mass spectrometry

Journal article

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Peer reviewed

Detection of SPM-1-producing Pseudomonas aeruginosa and class D β-lactamase-producing Acinetobacter baumannii isolates by use of liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization-time of flight mass spectrometry

Cecilia G Carvalhaes, Rodrigo Cayô, Diego M Assis, Evelin R Martins, Luiz Juliano, Maria A Juliano and Ana C Gales

Journal of clinical microbiology, Vol.51(1), pp.287-290

01/2013

DOI: 10.1128/JCM.02365-12

PMCID: PMC3536220

PMID: 23100344

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Abstract

This study evaluates the accuracy of liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) for detecting carbapenem hydrolytic activity among SPM-1-, GIM-1-, and GES-5-producing Pseudomonas aeruginosa isolates and OXA-143-, IMP-10-, and OXA-58-producing Acinetobacter baumannii isolates. Class A and B carbapenemase activities were rapidly detected by MALDI-TOF in a 2-h assay. However, an extended incubation time was necessary for detection of carbapenem-hydrolyzing class D β-lactamase (CHDL) activity in Acinetobacter spp.

Acinetobacter baumannii - chemistry

Acinetobacter baumannii - enzymology

Acinetobacter baumannii - isolation & purification

Bacteriological Techniques - methods

beta-Lactamases - secretion

Humans

Pseudomonas aeruginosa - chemistry

Pseudomonas aeruginosa - enzymology

Pseudomonas aeruginosa - isolation & purification

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods

Details

Title: Subtitle: Detection of SPM-1-producing Pseudomonas aeruginosa and class D β-lactamase-producing Acinetobacter baumannii isolates by use of liquid chromatography-mass spectrometry and matrix-assisted laser desorption ionization-time of flight mass spectrometry
Creators: Cecilia G Carvalhaes - Universidade Federal de São Paulo
Rodrigo Cayô - Universidade Federal de São Paulo
Diego M Assis - Universidade Federal de São Paulo
Evelin R Martins - Universidade Federal de São Paulo
Luiz Juliano - Universidade Federal de São Paulo
Maria A Juliano - Universidade Federal de São Paulo
Ana C Gales - Universidade Federal de São Paulo
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.51(1), pp.287-290
DOI: 10.1128/JCM.02365-12
PMID: 23100344
PMCID: PMC3536220
ISSN: 0095-1137
eISSN: 1098-660X
Language: English
Date published: 01/2013
Academic Unit: Pathology
Record Identifier: 9984183992602771

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