Detection of a biomarker for Alzheimer's disease from synthetic and clinical samples using a nanoscale optical biosensor

Amanda J Haes; Lei Chang; William L Klein; Richard P Van Duyne

doi:10.1021/ja044087q

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Journal article

Detection of a biomarker for Alzheimer's disease from synthetic and clinical samples using a nanoscale optical biosensor

Amanda J Haes, Lei Chang, William L Klein and Richard P Van Duyne

Journal of the American Chemical Society, Vol.127(7), pp.2264-2271

02/23/2005

DOI: 10.1021/ja044087q

PMID: 15713105

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Abstract

A nanoscale optical biosensor based on localized surface plasmon resonance (LSPR) spectroscopy has been developed to monitor the interaction between the antigen, amyloid-beta derived diffusible ligands (ADDLs), and specific anti-ADDL antibodies. Using the sandwich assay format, this nanosensor provides quantitative binding information for both antigen and second antibody detection that permits the determination of ADDL concentration and offers the unique analysis of the aggregation mechanisms of this putative Alzheimer's disease pathogen at physiologically relevant monomer concentrations. Monitoring the LSPR-induced shifts from both ADDLs and a second polyclonal anti-ADDL antibody as a function of ADDL concentration reveals two ADDL epitopes that have binding constants to the specific anti-ADDL antibodies of 7.3 x 10(12) M(-1) and 9.5 x 10(8) M(-1). The analysis of human brain extract and cerebrospinal fluid samples from control and Alzheimer's disease patients reveals that the LSPR nanosensor provides new information relevant to the understanding and possible diagnosis of Alzheimer's disease.

Ligands

Biomarkers - metabolism

Surface Plasmon Resonance

Antibodies - metabolism

Nanotechnology - methods

Humans

Biomarkers - analysis

Antigens - metabolism

Alzheimer Disease - cerebrospinal fluid

Brain - metabolism

Peptide Fragments - immunology

Alzheimer Disease - metabolism

Amyloid beta-Peptides - metabolism

Amyloid beta-Peptides - analysis

Amyloid beta-Peptides - cerebrospinal fluid

Antibodies - immunology

Antibody Formation

Amyloid beta-Peptides - immunology

Antigens - immunology

Biosensing Techniques - methods

Biomarkers - cerebrospinal fluid

Details

Title: Subtitle: Detection of a biomarker for Alzheimer's disease from synthetic and clinical samples using a nanoscale optical biosensor
Creators: Amanda J Haes - Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208-3113, USA
Lei Chang
William L Klein
Richard P Van Duyne
Resource Type: Journal article
Publication Details: Journal of the American Chemical Society, Vol.127(7), pp.2264-2271
DOI: 10.1021/ja044087q
PMID: 15713105
NLM abbreviation: J Am Chem Soc
ISSN: 0002-7863
eISSN: 1520-5126
Publisher: United States
Language: English
Date published: 02/23/2005
Academic Unit: Chemistry
Record Identifier: 9983985871102771

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