Detection of a murine coronavirus nonstructural protein encoded in a downstream open reading frame

Julian L Leibowitz; Stanley Perlman; George Weinstock; James R Devries; Carol Budzilowicz; Jane M Weissemann; Susan R Weiss

doi:10.1016/0042-6822(88)90631-9

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Detection of a murine coronavirus nonstructural protein encoded in a downstream open reading frame

Journal article

Open access

Peer reviewed

Detection of a murine coronavirus nonstructural protein encoded in a downstream open reading frame

Julian L Leibowitz, Stanley Perlman, George Weinstock, James R Devries, Carol Budzilowicz, Jane M Weissemann and Susan R Weiss

Virology, Vol.164(1), pp.156-164

05/1988

DOI: 10.1016/0042-6822(88)90631-9

PMCID: PMC7131066

PMID: 2834866

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Abstract

Mouse hepatitis virus (MHV) gene 5 contains two open reading frames. We have expressed the second open reading frame of this gene (gene 5 ORF 2) in an Escherichia coli expression system. This system utilized a plasmid which contained the promoter and the first 36 codons of the recA gene fused in frame with the MHV gene 5 ORF 2, which is fused in turn to the beta-galactosidase gene. The protein product of this gene fusion was used to raise antibody to gene 5 ORF 2. The specificity of the antibody was verified by immunoprecipitation of the in vitro transcribed and translated protein product of gene 5 ORF 2. The second reading frame of MHV gene 5 was shown to be expressed during the course of infection by immunocytochemistry and radioimmunoprecipitation using the antibody raised against the E. coli fusion protein and by two-dimensional gel electrophoresis.

Genes

Plasmids

Genes, Viral

Recombinant Fusion Proteins - genetics

Viral Proteins - genetics

Genetic Vectors

Murine hepatitis virus - genetics

Details

Title: Subtitle: Detection of a murine coronavirus nonstructural protein encoded in a downstream open reading frame
Creators: Julian L Leibowitz - Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, Houston 77225
Stanley Perlman
George Weinstock
James R Devries
Carol Budzilowicz
Jane M Weissemann
Susan R Weiss
Resource Type: Journal article
Publication Details: Virology, Vol.164(1), pp.156-164
DOI: 10.1016/0042-6822(88)90631-9
PMID: 2834866
PMCID: PMC7131066
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: United States
Grant note: NS 21954 / NINDS NIH HHS AI 17418 / NIAID NIH HHS NS 20834 / NINDS NIH HHS
Language: English
Date published: 05/1988
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777474202771

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