Detection of carbapenemase activity directly from blood culture vials using MALDI-TOF MS: a quick answer for the right decision

Cecilia G Carvalhaes; Rodrigo Cayô; Marina F Visconde; Talita Barone; Eliete A M Frigatto; Debora Okamoto; Diego M Assis; Luiz Juliano; Antonia M O Machado; Ana C Gales

doi:10.1093/jac/dku094

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Detection of carbapenemase activity directly from blood culture vials using MALDI-TOF MS: a quick answer for the right decision

Journal article

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Detection of carbapenemase activity directly from blood culture vials using MALDI-TOF MS: a quick answer for the right decision

Cecilia G Carvalhaes, Rodrigo Cayô, Marina F Visconde, Talita Barone, Eliete A M Frigatto, Debora Okamoto, Diego M Assis, Luiz Juliano, Antonia M O Machado and Ana C Gales

Journal of antimicrobial chemotherapy, Vol.69(8), pp.2132-2136

08/2014

DOI: 10.1093/jac/dku094

PMID: 24722840

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Abstract

Recently, matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was successfully applied for the detection of carbapenemase activity directly from Gram-negative colonies. Based on this principle, we evaluated the performance of MALDI-TOF MS for rapid detection of carbapenemase activity directly from positive blood culture vials. A total of 100 blood culture vials were randomly selected. MALDI-TOF MS carbapenemase assay results were confirmed by the detection of carbapenemase-encoding genes. A total of 110 bacterial isolates were recovered. The MALDI-TOF MS carbapenemase assay identified 21 of 29 (72.4%) of the carbapenemase-producing isolates directly from the blood culture vials, especially those encoding KPC-2 (100%) and SPM-1 (100%), after a 4 h incubation period. Although the majority of OXA-23-producing Acinetobacter baumannii isolates were not identified on day 1, all isolates were identified as carbapenemase producers directly from the colony on the next day. The MALDI-TOF MS carbapenemase assay is a feasible and rapid test to identify carbapenemase activity directly from blood culture vials. It may contribute to faster readjustment of empirical antimicrobial therapy and implementation of infection control measures.

Acinetobacter baumannii - enzymology

Acinetobacter baumannii - isolation & purification

Bacterial Proteins - blood

beta-Lactamases - blood

Humans

Klebsiella pneumoniae - enzymology

Klebsiella pneumoniae - isolation & purification

Pseudomonas aeruginosa - enzymology

Pseudomonas aeruginosa - isolation & purification

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods

Details

Title: Subtitle: Detection of carbapenemase activity directly from blood culture vials using MALDI-TOF MS: a quick answer for the right decision
Creators: Cecilia G Carvalhaes - Universidade Federal de São Paulo
Rodrigo Cayô - Universidade Federal de São Paulo
Marina F Visconde - Universidade Federal de São Paulo
Talita Barone - Universidade Federal de São Paulo
Eliete A M Frigatto - Hospital São Paulo
Debora Okamoto - Universidade Federal de São Paulo
Diego M Assis - Universidade Federal de São Paulo
Luiz Juliano - Universidade Federal de São Paulo
Antonia M O Machado - Hospital São Paulo
Ana C Gales - Universidade Federal de São Paulo
Resource Type: Journal article
Publication Details: Journal of antimicrobial chemotherapy, Vol.69(8), pp.2132-2136
DOI: 10.1093/jac/dku094
PMID: 24722840
ISSN: 0305-7453
eISSN: 1460-2091
Language: English
Date published: 08/2014
Academic Unit: Pathology
Record Identifier: 9984185277102771

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