Journal article
Detection of immunoglobulin/c-myc recombinations in mice that are resistant to plasmacytoma induction
Cancer research (Chicago, Ill.), Vol.56(2), pp.419-423
01/15/1996
PMID: 8542601
Abstract
Interchromosomal recombinations between c-myc and immunoglobulin sequences can be found in preneoplastic lesions (oil granulomata) during pristane-induced plasmacytoma development in susceptible BALB/cAn mice. In this study we used a more sensitive approach, hybridization-enriched templates with nested PCR, to detect microclones with Ig alpha/c-myc recombinations in oil granulomata of susceptible and resistant mice. Recombinations were detected in as many as 73% (32/44) of plasmacytoma-susceptible BALB/cAn mice 30 days after an injection of pristane. Mice that are resistant to plasmacytoma induction can also harbor recombination-positive cells, but these are less frequent [2/20 DBA/2N, 8/20 (BALB/cAn x DBA/2N)F1, hereafter called CD2F1]. The clones in DBA/2N mice were small (< 400 cells), whereas in BALB/cAn, the oil granuloma harbored up to several thousand of these cells. We conclude that the molecular machinery for generating characteristic interchromosomal recombinations can be found in all strains of mice. Both the frequency of generating Ig alpha/c-myc recombinations and the expansion of recombination-positive cells are greater in susceptible mice than in resistant strains.
Details
- Title: Subtitle
- Detection of immunoglobulin/c-myc recombinations in mice that are resistant to plasmacytoma induction
- Creators
- Jürgen R Müller - Laboratory of Genetics, National Cancer Institute, NIH, Bethesda, Maryland 20892-4255, USAGary M JonesMichael PotterSiegfried Janz
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.56(2), pp.419-423
- PMID
- 8542601
- NLM abbreviation
- Cancer Res
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Language
- English
- Date published
- 01/15/1996
- Academic Unit
- Pathology
- Record Identifier
- 9984083996602771
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