Journal article
Detection of lipid radicals using EPR
Antioxidants & redox signaling, Vol.6(3), pp.631-638
06/2004
DOI: 10.1089/152308604773934396
PMID: 15130290
Abstract
Cells oxidize molecules to generate energy and to make the materials to build and support the structures and functions needed for life. However, unwanted oxidations can damage these same structures and impair function. Lipids (the lipids in membranes and lipoproteins) are targets of unwanted oxidations. The primary mechanism of these oxidations is free radical-mediated chain reactions. Here we provide an overview of how electron paramagnetic resonance (EPR) can be used to detect the free radicals formed during lipid peroxidation. Although direct detection of lipid-derived radicals has been accomplished, the approach is not feasible for detecting these radicals in cells. Spin trapping with alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone and 5,5-dimethyl-pyrroline-1-oxide has provided the most information on cellular lipid peroxidation. We present some considerations for successful detection of lipid radicals by EPR.
Details
- Title: Subtitle
- Detection of lipid radicals using EPR
- Creators
- Sujatha Venkataraman - Free Radical and Radiation Biology & ESR Facility, The University of Iowa, Iowa City, IA 52242-1101, USAFreya Q SchaferGarry R Buettner
- Resource Type
- Journal article
- Publication Details
- Antioxidants & redox signaling, Vol.6(3), pp.631-638
- Publisher
- United States
- DOI
- 10.1089/152308604773934396
- PMID
- 15130290
- ISSN
- 1523-0864
- eISSN
- 1557-7716
- Grant note
- CA-81090 / NCI NIH HHS CA84462 / NCI NIH HHS CA-66081 / NCI NIH HHS
- Language
- English
- Date published
- 06/2004
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984047650902771
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