Detection of posttranslational modifications of replication protein A

Cathy S Hass; Ran Chen; Marc S Wold

doi:10.1007/978-1-62703-032-8_15

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Journal article

Detection of posttranslational modifications of replication protein A

Cathy S Hass, Ran Chen and Marc S Wold

Methods in molecular biology (Clifton, N.J.), Vol.922, pp.193-204

2012

DOI: 10.1007/978-1-62703-032-8_15

PMCID: PMC4629249

PMID: 22976188

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Abstract

Replication Protein A (RPA) is a single-strand DNA-binding protein that is found in all eukaryotes. RPA is subjected to multiple posttranslational modifications including serine- and threonine-phosphorylation, poly-ADP ribosylation, and SUMOylation. These modifications are believed to regulate RPA activity through modulating interactions with DNA and partner proteins. This article describes two methods used to detect posttranslational modified RPA: immunofluorescence and immmuoblotting.

DNA-Binding Proteins - metabolism

Fluorescent Antibody Technique - methods

Replication Protein A - genetics

DNA Replication

Protein Processing, Post-Translational

Replication Protein A - metabolism

DNA-Binding Proteins - genetics

Molecular Biology - methods

Immunoblotting - methods

Details

Title: Subtitle: Detection of posttranslational modifications of replication protein A
Creators: Cathy S Hass - Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Ran Chen
Marc S Wold
Resource Type: Journal article
Publication Details: Methods in molecular biology (Clifton, N.J.), Vol.922, pp.193-204
DOI: 10.1007/978-1-62703-032-8_15
PMID: 22976188
PMCID: PMC4629249
NLM abbreviation: Methods Mol Biol
eISBN: 9781627030328; 1627030328
ISSN: 1064-3745
eISSN: 1940-6029
Publisher: United States
Grant note: R01 GM044721 / NIGMS NIH HHS
Language: English
Date published: 2012
Academic Unit: Radiation Oncology; Biochemistry and Molecular Biology
Record Identifier: 9984024521102771

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