Journal article
Detection of posttranslational modifications of replication protein A
Methods in molecular biology (Clifton, N.J.), Vol.922, pp.193-204
2012
DOI: 10.1007/978-1-62703-032-8_15
PMCID: PMC4629249
PMID: 22976188
Abstract
Replication Protein A (RPA) is a single-strand DNA-binding protein that is found in all eukaryotes. RPA is subjected to multiple posttranslational modifications including serine- and threonine-phosphorylation, poly-ADP ribosylation, and SUMOylation. These modifications are believed to regulate RPA activity through modulating interactions with DNA and partner proteins. This article describes two methods used to detect posttranslational modified RPA: immunofluorescence and immmuoblotting.
Details
- Title: Subtitle
- Detection of posttranslational modifications of replication protein A
- Creators
- Cathy S Hass - Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA, USARan ChenMarc S Wold
- Resource Type
- Journal article
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.922, pp.193-204
- DOI
- 10.1007/978-1-62703-032-8_15
- PMID
- 22976188
- PMCID
- PMC4629249
- NLM abbreviation
- Methods Mol Biol
- eISBN
- 9781627030328; 1627030328
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Publisher
- United States
- Grant note
- R01 GM044721 / NIGMS NIH HHS
- Language
- English
- Date published
- 2012
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984024521102771
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